CYTOLOGICAL STUDIES OF PRIMATE VAGINAL EPITHELIUM

这项提案将解决两个关键问题 了解阴道上皮的结构和生理。这些 1)阴道通透性屏障的性质是什么？ 上皮？以及2)阴道细胞表面的特性是什么 会影响细菌在细胞上的黏附吗？要测试 细胞间物质可能形成部分通透性的假说 将进行屏障、组织化学和细胞化学研究 证明脂类、糖偶联物和 人和猴阴道上皮中的溶酶体酶。结果将会是 可与其他角化中发现的局部化和 非角化性上皮。完整上皮细胞的通透性屏障将 通过注入电致密示踪分子(镧)进一步检查 和过氧化物酶)进入猴阴道固有层，随后 电子显微镜示踪剂进入上皮的过程。 将对局部应用的示踪剂进行类似的实验 检查表面细胞的渗透屏障。人阴道 在细胞培养系统中，上皮细胞将生长到融合，并且 该细胞的形态分化、细胞化学和渗透性 上皮细胞将通过类似的技术进行检查，并与 未培养的上皮细胞。研究细菌对培养物的粘附性 上皮细胞，最佳浓度的乳酸菌将被添加到 培养的上皮细胞及其黏附情况进行了定量。PH的影响， 温度、二价阳离子和细胞表面的修饰 将测量粘着时的碳水化合物。

This proposal will address two major questions critical to the understanding of the structure and physiology of vaginal epithelium. These are: 1) What is the nature of the permeability barrier in vaginal epithelium?, and 2) What are the properties of the vaginal cell surface that influence the adherence of bacteria to the cells? To test the hypothesis that intercellular substances may form part of the permeability barrier, histochemical and cytochemical studies will be conducted to demonstrate the nature and localization of lipids, glycoconjugates, and lysosomal enzymes in human and monkey vaginal epithelium. The results will be compared to localizations found in other keratinizing and non-keratinizing epithelia. Permeability barriers in intact epithelia will be further examined by injecting electro-dense tracer molecules (lanthanum and peroxidase) into the lamina propria of monkey vagina and following by electron microscopy the movement of the tracers into the epithelium. Similar experiments will be carried out with topically applied tracers to examine permeability barriers of the surface cells. Human vaginal epithelium will be grown to confluence in a cell culture system and the morphological differentiation, cytochemistry and permeability of this epithelium will be examined by similar techniques and compared to non-cultured epithelium. To study bacterial adherence to the cultured epithelium, optimal concentrations of Lactobacillus will be added to the cultured epithelium and their adherence quantitated. The effects of pH, temperature, divalentcations, and modification of cell surface carbohydrates on adherence will be measured.