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CYTOLOGICAL STUDIES OF PRIMATE VAGINAL EPITHELIUM

灵长类阴道上皮的细胞学研究

基本信息

批准号：
3312418
负责人：
BARRY F KING
金额：
$ 8.8万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-04-01 至 1986-03-31
项目状态：
已结题

项目摘要

This proposal will address two major questions critical to the understanding of the structure and physiology of vaginal epithelium. These are: 1) What is the nature of the permeability barrier in vaginal epithelium?, and 2) What are the properties of the vaginal cell surface that influence the adherence of bacteria to the cells? To test the hypothesis that intercellular substances may form part of the permeability barrier, histochemical and cytochemical studies will be conducted to demonstrate the nature and localization of lipids, glycoconjugates, and lysosomal enzymes in human and monkey vaginal epithelium. The results will be compared to localizations found in other keratinizing and non-keratinizing epithelia. Permeability barriers in intact epithelia will be further examined by injecting electro-dense tracer molecules (lanthanum and peroxidase) into the lamina propria of monkey vagina and following by electron microscopy the movement of the tracers into the epithelium. Similar experiments will be carried out with topically applied tracers to examine permeability barriers of the surface cells. Human vaginal epithelium will be grown to confluence in a cell culture system and the morphological differentiation, cytochemistry and permeability of this epithelium will be examined by similar techniques and compared to non-cultured epithelium. To study bacterial adherence to the cultured epithelium, optimal concentrations of Lactobacillus will be added to the cultured epithelium and their adherence quantitated. The effects of pH, temperature, divalentcations, and modification of cell surface carbohydrates on adherence will be measured.

这一建议将解决两个关键问题，了解阴道上皮的结构和生理学。这些 1）阴道中渗透屏障的性质是什么？上皮？，阴道细胞表面的性质是什么影响细菌附着在细胞上的机制测试假设细胞间物质可能构成渗透性的一部分，将进行屏障、组织化学和细胞化学研究，证明脂质、糖缀合物和人和猴阴道上皮中的溶酶体酶。结果将与其他角质化和非角化上皮。完整上皮中的渗透屏障将通过注入电致密示踪剂分子（镧和过氧化物酶）进入猴阴道的固有层，然后电子显微镜观察示踪剂进入上皮的运动。将用局部施用的示踪剂进行类似的实验，检查表面细胞的渗透屏障。人阴道上皮将在细胞培养系统中生长至汇合，形态分化，细胞化学和渗透性，将通过类似的技术检查上皮，并与非培养上皮。为了研究细菌对培养的上皮，将最佳浓度的乳酸杆菌添加到培养的上皮细胞及其粘附定量。 pH值的影响，温度、二价阳离子和细胞表面修饰将测量碳水化合物对粘附的影响。