POST-IMPLANTATION MODIFICATION OF THE SPIRAL ARTERIES

螺旋动脉的植入后改造

• 批准号: 3325108
• 负责人: BARRY F KING
• 金额: $ 15.39万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3325108
• 关键词: Macaca mulatta; collagen; connective tissue; histochemistry /cytochemistry; immunochemistry; lysosomes; nonsurgical revascularization; pregnancy circulation; pregnancy toxemia /hypertension; scanning electron microscopy; trophoblast; vascular endothelium; vascular smooth muscle

The blood supply to the primate placenta is established by the conversion of the muscular spiral arteries into dilated funnel- shaped uteroplacental arteries whose walls are composed mainly of trophoblast cells and connective tissue. These changes are thought to accommodate the greater volume of blood flow required by the developing conceptus, and to modify the normal maternal control mechanisms of these vessels. It has been observed that pregnancies complicated by preeclampsia alone, or preeclampsia with essential hypertension, structural modification of the arterial walls is less extensive and this apparently compromises uteroplacental perfusion. The key element in the conversion of the arteries is the migration of endovascular cells, thought to be trophoblast, into the spiral arteries. The endothelium is apparently removed and this is followed by the destruction of the muscular arterial wall and its replacement by trophoblast and connective tissue. There are many gaps in our understanding of the development of the uteroplacental arteries in primates especially the cellular mechanisms by which the invasion, destruction and replacement are carried out. The long- term objective of this proposal is the characterize the process of invasion and modification of the spiral arteries in the rhesus monkey, a species with many placental anatomical similarities to the human. Specific aims are 1) to define ultrastructurally and immunocytochemically the sequence of cellular interactions between endovascular trophoblast and endothelial cells during the initial invasion of the spiral arteries; 2) to determine the cellular mechanisms by which the endovascular trophoblast removes the maternal endothelium, penetrates the walls of the arteries and destroys the smooth muscle of the tunica media. At the present time, there is little information available on how this is accomplished in primates. Careful ultrastructural descriptions will be coupled with cytochemical studies to evaluate the role of lysosomal enzymes in this process, a role suggested by studies in other species; and 3) to determine, using immunocytochemical methods, the changes in the composition of the connective tissue components of the arterial wall during the conversion of the spiral arteries to the uteroplacental arteries.

灵长类动物胎盘的血液供应是由 肌性螺旋动脉转变为扩张的漏斗状动脉 形子宫胎盘动脉，其壁主要由 滋养层细胞和结缔组织。 这些变化是 被认为可以容纳更大的血液流量 需要的发展概念，并修改正常的 这些血管的母体控制机制。 已经 观察到妊娠合并单独的先兆子痫，或 子痫前期合并原发性高血压，结构改变 动脉壁的面积较小， 危及子宫胎盘灌注。 的关键要素 动脉的转化是血管内细胞的迁移， 被认为是滋养层的细胞进入螺旋动脉。 的 内皮细胞明显被去除，随后是 肌肉动脉壁的破坏和替代 滋养层和结缔组织。 在我们的世界里， 了解子宫胎盘动脉的发育 特别是灵长类动物的细胞机制， 进行入侵、破坏和替换。 很长的- 本提案的长期目标是表征 恒河猴螺旋动脉的侵犯和改变 猴子，一种与许多胎盘解剖学相似的物种， 人类 具体目标是：1）在超微结构上定义， 免疫细胞化学的细胞相互作用的顺序 血管内滋养层细胞和内皮细胞之间的关系 螺旋动脉的初始侵入; 2）确定细胞 血管内滋养层细胞去除 母体内皮，穿透动脉壁， 破坏了图尼卡的平滑肌。 现 时间，有很少的信息，这是如何 在灵长类动物中完成的。 仔细的超微结构描述 将与细胞化学研究相结合，以评估 溶酶体酶在这一过程中的作用，研究表明， 其他物种;和3）确定，使用免疫细胞化学 方法，结缔组织成分的变化 在螺旋转换期间动脉壁的成分 子宫胎盘动脉