HORMONAL CONTROL OF DELAYED IMPLANTATION

延迟着床的荷尔蒙控制

• 批准号: 3310512
• 负责人: RODNEY A MEAD
• 金额: $ 8.24万
• 依托单位: UNIVERSITY OF IDAHO
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310512
• 关键词: chemical structure; corpus luteum; early embryonic stage; embryo /fetus; glucose metabolism; growth /development; high performance liquid chromatography; hormone regulation /control mechanism; mammalian embryology; ovariectomy; pituitary gonadal axis; progesterone; radiotracer

The primary hypothesis to be tested is that a compound(s) of luteal origin acts in conjunction with progesterone to initiate renewed embryonic development and implantation in the ferret and in a species exhibiting an obligate delay of implantation. The first objective is to determine the chemical nature (lipid or protein) of the ovarian factor, other than progesterone, which induces implantation in ferrets. This will be accomplished by extracting the proteins and lipids from ovaries obtained from ferrets on the eighth day of pregnancy and testing the unfractionated and fractioned lipids and proteins for their ability to induce implantation in ovariectomized (Day 6) progesterone-treated ferrets and in intact spotted skunks with delayed implanting blastocysts. After the general chemical nature of the ovarian implantation factor is determined, attempts will be made to further characterize or identify the compound(s). Attempts will be made to verify that corpora lutea (CL) are a source of the compound, by culturing day eight CL obtained from ferrets with labeled precursors and then subjecting the conditioned culture medium to the same fractionation procedure which produced a biologically active product capable of inducing implantation. Lastly, an attempt will be made to determine whether the ovarian implantation factor stimulates embryonic development by acting directly on the blastocyst or indirectly through its action on the uterus. This will be accomplished by flushing the blastocysts from the uterus in situ and comparing changes in blastocyst diameter, 14C-glucose utilization and synthesis of protein, RNA, and DNA after short-term culture in the presence or absence of varying concentrations of the partially purified ovarian factor. The ovarian factor will likewise be administered to these same animals (whose uteri now lack blastocysts) for several days, and the uteri examined for changes in incorporation of 3H-uridine and 3H-thymidine into RNA and DNA. The long term goal of the proposed research is to define the mechanisms by which hormones of the anterior pituitary and ovary interact in controlling the cessation and the subsequent reinitiation of embryonic development in mammals exhibiting an obligate delay of implantation.

待检验的主要假设是黄体来源的化合物 与孕酮共同作用，启动更新的胚胎 在雪貂和表现出 延迟着床。 第一个目标是确定 卵巢因子的化学性质（脂质或蛋白质），除 孕酮，它能诱导雪貂的着床。 这将是 通过从卵巢中提取蛋白质和脂质， 从雪貂怀孕第八天， 并将脂质和蛋白质分级以诱导植入 在卵巢切除（第6天）的炔雌醇治疗的雪貂和完整的 带有延迟着床囊胚的斑点臭鼬。 后将军 卵巢植入因子的化学性质被确定， 以进一步表征或鉴定化合物。 尝试 将进行验证，以验证黄体（CL）是 化合物，通过培养从标记的雪貂中获得的第八天CL 前体，然后使条件培养基经受相同的条件， 产生生物活性产物的分馏过程 能够诱导植入。 最后，将尝试 确定卵巢植入因子是否刺激胚胎 通过直接作用于胚泡或间接通过其 对子宫的作用。 这将通过刷新 原位子宫囊胚，比较囊胚 直径、14 C-葡萄糖利用和蛋白质、RNA和DNA的合成 在存在或不存在不同的 部分纯化的卵巢因子的浓度。 卵巢 因子将同样地施用给这些相同的动物（其子宫现在 缺乏胚泡）几天，检查子宫中 3H-尿苷和3H-胸苷掺入RNA和DNA。 长 拟议研究的长期目标是确定机制， 垂体前叶和卵巢的激素相互作用， 胚胎发育的停止和随后的重新开始， 表现出专性着床延迟的哺乳动物。