HORMONAL CONTROL OF DELAYED IMPLANTATION

这项研究的长期目标是通过以下方式定义这些机制 哪种光周期、下丘脑、垂体和 卵巢在控制停滞和再启动中的相互作用 一个物种的胚胎发育和植入 刻意延迟植入。这项提议特别寻求 提供更多证据来支持语料库 家养雪貂等芥子状食肉动物和 斑点臭鼬分泌一种必需的蛋白质化合物 用于诱导胚泡着床。我建议进一步 雪貂黄体着床的纯化及化学特性研究 以及确定哪种营养因子(S)刺激其 体外合成和分泌。然后我提议证明 通过雪貂和斑点臭鼬的被动免疫， 抗LIF抗体将抑制着床。另一项实验 旨在确定怀孕的哪几天或 假孕LIF在雪貂体内分泌。我还提议 进一步验证LIF只由雪貂的CL和TO产生 探讨其亚细胞分布和分泌方式 免疫细胞化学。另一系列实验旨在 确定LIF是否作用于子宫和/或胚泡，并 提供证据证明存在与此相关的受体位点 化合物。这将通过研究特定的绑定来实现 ~(125)I-LIF对子宫和胚泡质膜的作用。进一步 具体受体位置的演示将通过以下方式完成 ~(125)I)-LIF-受体络合物的提取 通过电泳法和放射自显影。最后一套 实验旨在确定激光诱导因子的具体效果 通过测量cAMP的变化等对靶器官的影响， 子宫和/或胚泡中蛋白质、RNA和DNA的合成。

The long term goal of this research is to define the mechanisms by which photoperiod, hormones of the hypothalamus, pituitary and ovary interact in controlling cessation and reinitiation of embryonic development and implantation in a species exhibiting obligate delay of implantation. This proposal specifically seeks to Provide additional evidence to support the theory that corpora lutea of mustelid carnivores such as the domesticated ferret and spotted skunk secrete a proteinacious compound that is essential for induction of blastocyst implantation. I propose to further purify and chemically characterize the ferret luteal implantation factor (LIF) and to determine which trophic factor(s) stimulate its synthesis and secretion in vitro. I then propose to demonstrate by passive immunization of ferrets and spotted skunks, that antibodies to LIF will inhibit implantation. Another experiment is designed to determine on which days of pregnancy or pseudopregnancy LIF is secreted in ferrets. I also propose to further verify that LIF is only produced by CL of ferrets and to explore its subcellular distribution and mode of secretion using immunocytochemistry. Another series of experiments is designed to determine whether LIF acts on the uterus and or blastocysts and to provide evidence for the presence of receptor sites to this compound. This will be accomplished by studying specific binding of (125I)-LIF to uterine and blastocyst plasma membranes. Further demonstration of specific receptor sites will be accomplished by extraction of cross-linked (125I)-LIF-receptor complexes, followed by electrophoresis and autoradiography. The last set of experiments is designed to determine the specific effects of LIF on its target organs by measuring such things as changes in cAMP, protein, RNA and DNA synthesis in the uterus and or blastocysts.