BRAIN MECHANISMS OF REPRODUCTIVE BEHAVIOR

生殖行为的大脑机制

• 批准号: 3310418
• 负责人: Donald W. Pfaff
• 金额: $ 14.13万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1986-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310418
• 关键词: autoradiography; brain stem; denervation; electromyography; electrophysiology; electrostimulus; estrogens; experimental brain lesion; female; histochemistry /cytochemistry; hormone regulation /control mechanism; immunochemistry; mesencephalon; microinjections; neural transmission; neuroendocrine system; neuropsychology; progesterone; psychophysiology; radiotracer; reticulospinal tract; sex behavior; sex hormones; single cell analysis; spinal cord mapping

We will close the gaps in our knowledge of the neural mechanisms for lordosis behavior, and estrogen and progesterone effects on it in female rats. We aim to provide a complete neural and endocrine explanation of this component of reproductive behavior. I. To characterize the estrogen effect on hypothalamic cells more precisely we will use a quantitative application of steroid hormone autoradiography to test ideas of the estradiol uptake in specific cells: Is it active (vs. random, Poisson)? Is it quantal (vs. continuous)? Combining autoradiography with other techniques will tell us more about specific estrogen-binding neurons. Where do they project (autoradiography combined with HRP)? What important substances do they contain (autoradiography combined with HRP)? What important substances do they contain (autoradiography combined with immunocytochemistry)? Microinjection experiments will reveal the nature of behaviorally important ventromedial hypothalamic (VMH) actions. Are they electrical, secretory, or both? Single unit recording in VMH will characterize progesterone effects on these cells. II. Hypothalamic output must be registered in the midbrain. Electrical stimulation and microinjection of cells in and adjacent to the midbrain central grey will test the electrical and possible (modulatory) secretory actions here for their importance for lordosis behavior. Possible (synergistic) interactions of secretory with electrical processes in central grey will also be tested. LHRH in midbrain central grey can facilitate lordosis. We will analyze the relation of this behavioral LHRH effect to (a) LHRH chemical structure, (b) time of day, and (c) steroid hormone levels. III. Tracts descending from brainstem to spinal cord important for lordosis are the medullary (lateral) reticulospinal tract (RST) and the lateral vestibulospinal tract (LVST). We will analyze the RST role by chemical destruction of medullary reticular neurons and by electrical stimulation. The "trading relations" of LVST electrical signals with hormone levels will be studied by systematically varying LVST stimulation current, estradiol dose and progesterone dose. Finally, normal electrical activity of single units during lordosis and other behaviors will be demonstrated by chronic recording from individual RST and (in separate experiments) LVST neurons.

我们将弥合我们对前凸神经机制知识的空白。 雌性大鼠的行为，以及雌激素和孕激素对其的影响。我们的目标是 对这一成分提供完整的神经和内分泌解释 生殖行为。 I.为了更准确地描述雌激素对下丘脑细胞的影响 会用定量应用类固醇激素放射自显影来检测 关于在特定细胞中摄取雌二醇的想法：它是活跃的(与随机的， 泊松)？它是量化的(与连续的)吗？将放射自显影与其他 技术将告诉我们更多关于特定雌激素结合神经元的信息。在哪里？ 他们投射(放射自显影结合HRP)？重要的物质有什么作用 它们包含(放射自显影结合HRP)？重要的物质有什么作用 它们包含(放射自显影结合免疫细胞化学)？ 微量注射实验将揭示行为重要的本质 下丘脑腹内侧(VMH)活动。它们是电的，分泌物的，还是 两个都要？在VMH中的单单位记录将表征孕酮对 这些细胞。 下丘脑的输出必须在中脑登记。电气 刺激和微量注射中脑中央及其附近的细胞 格雷将在这里测试电和可能的(调制)分泌作用 因为它们对前凸行为的重要性。可能的(协同)互动 中央灰色的分泌物与电过程也将进行测试。 中脑中央灰质的LHRH可促进前凸。我们将分析 这种行为LHRH效应与(A)LHRH化学结构、(B)时间的关系 以及(C)类固醇激素水平。 III.从脑干下行到脊髓的束对前凸很重要 延髓(外侧)网状脊髓束(RST)和外侧 前庭脊髓束(LVST)。我们将从化学角度分析RST的作用 延髓网状神经元的破坏和电刺激。这个 LVST电信号与激素水平的“交易关系”将是 通过系统地改变LVST刺激电流、雌二醇剂量和 黄体酮剂量。最后，单台机组的正常电活动 前凸和其他行为将通过慢性记录从 单独的RST和(在单独的实验中)LVST神经元。