STUDIES OF MOUSE SPERMATOGENESIS AND FERTILIZATION

拟议的研究有两个具体目标。 第一个是定义 t突变的生理效应导致扭曲的 （非孟德尔）从雄性小鼠传递突变。 这些 研究-测定cAMP含量、腺苷酸环化酶和cAMP 磷酸二酯酶活性和cAMP依赖性蛋白磷酸化 模式-将在携带完整t突变的精子上进行， 在那些携带近端部分缺失或远端部分缺失的 T/T复合体。 完成这些比较研究将使我们能够 证实了我们的假设，即异常传输是由 cAMP水平异常，这反过来又导致异常的 蛋白质磷酸化 使用部分缺失的t突变染色体 应该能让我们绘制出控制这些生理变化的因素 参数 第二个具体目标是检验我们的假设， 与T/t复合体相关的表型综合征由 与t突变相关的独特H-2单倍型。 在这个系列中 我们将把携带野生型H-2序列的质粒注射到 t/t合子，并确定插入H-2DNA对胚胎的影响 致死率和男性传播频率。

The proposed research has two specific aims. The first is to define the physiological effects of t mutations which result in the distorted (non-Mendelian) transmission of the mutations from male mice. These studies - determining cAMP content, adenylate cyclase and cAMP phosphodiesterase activities and cAMP-dependent protein phosphorylation patterns - will be done on spermatozoa which carry complete t mutations and on those which carry either proximal partial or distal partial deletions of the T/t complex. Completion of these comparative studies will enable us to confirm our hypothesis that the abnormal transmissions are caused by aberrant levels of cAMP which, in turn, result in abnormal patterns of protein phosphorylation. Use of the partially deleted t mutant chromosomes should enable us to map the factors controlling these physiological parameters. The second specific aim is a test of our hypothesis that the phenotypic syndromes associated with the T/t complex are controlled by the unique H-2 haplotypes which are linked to the t mutations. In this series of studies we will inject plasmids carrying wild-type H-2 sequences into t/t zygotes and determine the effect of the inserted H-2 DNA on embryo lethality and male transmission frequency.