STUDIES OF MOUSE SPERMATOGENESIS AND FERTILIZATION

这项拟议的研究有两个具体目标。第一种方法是定义 导致畸形的t突变的生理效应 (非孟德尔式)雄性小鼠的突变传播。这些 研究-测定cAMP含量、腺苷环化酶和cAMP 磷酸二酯酶活性与cAMP依赖蛋白磷酸化 模式-将在携带完全t突变和 关于携带近端部分或远端部分缺失的那些 T/T复合体。完成这些比较研究将使我们能够 确认我们的假设，即异常传播是由 CAMP的异常水平，进而导致异常的模式 蛋白质磷酸化。利用部分缺失的t突变染色体 应该使我们能够绘制出控制这些生理因素的地图 参数。第二个具体目标是测试我们的假设，即 与T/t复合体相关的表型综合征由 与t突变相关的独特的H-2单倍型。在本系列中 我们将把携带野生型H-2序列的质粒注射到 T/t受精卵和检测插入H-2 DNA对胚胎的影响 致命性和男性传播频率。

The proposed research has two specific aims. The first is to define the physiological effects of t mutations which result in the distorted (non-Mendelian) transmission of the mutations from male mice. These studies - determining cAMP content, adenylate cyclase and cAMP phosphodiesterase activities and cAMP-dependent protein phosphorylation patterns - will be done on spermatozoa which carry complete t mutations and on those which carry either proximal partial or distal partial deletions of the T/t complex. Completion of these comparative studies will enable us to confirm our hypothesis that the abnormal transmissions are caused by aberrant levels of cAMP which, in turn, result in abnormal patterns of protein phosphorylation. Use of the partially deleted t mutant chromosomes should enable us to map the factors controlling these physiological parameters. The second specific aim is a test of our hypothesis that the phenotypic syndromes associated with the T/t complex are controlled by the unique H-2 haplotypes which are linked to the t mutations. In this series of studies we will inject plasmids carrying wild-type H-2 sequences into t/t zygotes and determine the effect of the inserted H-2 DNA on embryo lethality and male transmission frequency.