CONTROL OF LUTEINIZING HORMONE BIOSYNTHESIS

黄体生成素生物合成的控制

• 批准号: 3311142
• 负责人: GARY L JACKSON
• 金额: $ 11.63万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311142
• 关键词: carbohydrate structure; chemical group; cyclic nucleoside monophosphate; estrogens; estrus; glucose metabolism; glycosyltransferase; hormone regulation /control mechanism; luteinizing hormone; peptide hormone biosynthesis; pituitary gland; radioimmunoassay; radionuclide double label; scintillation counter; secretion; sex hormones

We will investigate biosynthesis by monitoring incorporation of radiolabelled precursors into LH by dispersed rat anterior pituitary cells. Newly synthesized LH will be isolated by immunoprecipitation with specific antibodies. The effects of estrogen and progesterone on LH biosynthesis will be determined in this system. Also the relationships between synthesis and release will be studied in an attempt to determine if these two steps of secretion are tightly coupled. The effect of GnRH on the size of LH will be monitored by comparing LH synthesized in the presence of GnRH with that synthesized in the absence of GnRH.

我们将通过监测放射性标记的 前体转化为LH。 新合成 将通过特异性抗体免疫沉淀分离LH。 的 雌激素和孕激素对LH生物合成的影响将在 这系统. 此外，合成和释放之间的关系将是 研究试图确定这两个分泌步骤是否紧密相关， 耦合。 通过比较LH，监测GnRH对LH大小的影响。 在存在GnRH的情况下合成的与在不存在GnRH的情况下合成的相比， 促性腺激素释放激素