FIELD-FLOW FRACTIONATION IN BIOCHEMICAL ANALYSIS

生物样品的分级和精确的大小表征 胶体和高分子量的大分子将被 使用沉降FFF的方法进行，并且其中 必要时，流量FFF。 通过制定具体程序， 各种各样的样品类型，其分离/表征 使用传统方法提出了挑战，该项目 将寻求增加FFF方法的实用性， 生物学特性研究。 沉降FFF与准弹性光的结合 散射对分子量有特别的希望 表征和粒度测定 密度未知的材料的分布。 的该模式 操作将进行研究，以使准确的表征 球形病毒、乳剂和脂质体。 空间FFF将应用于细胞的大小分选，由此 将努力使表面粘附最小化。 血小板将 为了将大小与 生理活性，HeLa细胞将根据 大小，以实现粒子的同步种群。 将努力使分馏类型特定 通过所需细胞的选择性聚集。 更换 电引力场对沉降场的影响将是 结合细胞分离进行探索。

The fractionation and accurate size characterization of biological colloids and macromolecules of high molecular weight will be pursued using the methods of sedimentation FFF, and where necessary, flow FFF. By developing specific procedures for a wide variety of sample types, whose separation/characterization presents challenges using conventional methodology, the project will seek to increase the usefulness of the FFF methods in biological characterization work. The combination of sedimentation FFF and quasi elastic light scattering holds particular promise for molecular weight characterization and the determination of particle size distributions of materials with unknown density. This mode of operation will be studied to enable the accurate characterization of spherical viruses, emulsions and liposomes. Steric FFF will be applied to the size-sorting of cells, whereby efforts will be made to minimize surface adhesion. Platelets will be fractionated for the purpose of correlating size with physiological activity, and HeLa cells will be sorted according to size in order to achieve synchronous populations of particles. Efforts will be made to render the fractionation type-specific through selective aggregation of desired cells. The replacement of the sedimentation field by an electrogravitational field will be explored in conjunction with cell separation.