FIELD-FLOW FRACTIONATION IN BIOCHEMICAL ANALYSIS

生物样品的分级和精确的粒度表征 胶体和高分子量的大分子将被 使用沉淀FFF的方法进行的，以及在哪里 必要的，流畅的FFF。通过制定具体的程序来实现 种类繁多的样品，其分离/表征 使用传统方法提出挑战，该项目 将寻求增加FFF方法在以下方面的实用性 生物表征工作。 沉降场与准弹性光的结合 散射对分子量具有特殊的前景 颗粒大小的表征和测定 密度未知的材料的分布。这种模式 将研究操作以使准确的表征成为可能 球形病毒、乳剂和脂质体。 立体FFF将应用于细胞的大小分类，从而 将努力将表面粘连降至最低。血小板会 为了将大小与关联的目的而进行细分 生理活性，HeLa细胞将按照 大小以实现粒子的同步种群。 将努力使分馏类型特定 通过选择性地聚集所需的细胞。接班人 沉降场的电引力场将是 结合细胞分离进行了探索。

The fractionation and accurate size characterization of biological colloids and macromolecules of high molecular weight will be pursued using the methods of sedimentation FFF, and where necessary, flow FFF. By developing specific procedures for a wide variety of sample types, whose separation/characterization presents challenges using conventional methodology, the project will seek to increase the usefulness of the FFF methods in biological characterization work. The combination of sedimentation FFF and quasi elastic light scattering holds particular promise for molecular weight characterization and the determination of particle size distributions of materials with unknown density. This mode of operation will be studied to enable the accurate characterization of spherical viruses, emulsions and liposomes. Steric FFF will be applied to the size-sorting of cells, whereby efforts will be made to minimize surface adhesion. Platelets will be fractionated for the purpose of correlating size with physiological activity, and HeLa cells will be sorted according to size in order to achieve synchronous populations of particles. Efforts will be made to render the fractionation type-specific through selective aggregation of desired cells. The replacement of the sedimentation field by an electrogravitational field will be explored in conjunction with cell separation.