BIOMEDICAL SEPARATIONS--FIELD FLOW FRACTIONATION

生物医学分离——场流分离

• 批准号: 2391776
• 负责人: Karin D Caldwell
• 金额: $ 11.21万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-02-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2391776
• 关键词: bacteria; blood lipoprotein; chromatography; colloids; erythrocytes; liposomes; macromolecule; method development

DESCRIPTION: The main objective of the proposed work is to develop field-flow fractionation (FFF) and its various sub-techniques into increasingly powerful tools for the separation and analysis of complex colloidal and macromolecular materials of biological and pharmaceutical origin. Theoretical and experimental studies will be combined to exploit the unique capabilities of FFF to fractionate and characterize high molecular weight materials relevant to the health sciences. The proposed work will be divided into several categories. In the first category, the investigator will pursue the optimization of FFF to yield higher speed, resolution, and convenience. He will convert optimization theory to simple laboratory guidelines. He will also study fundamental colloidal phenomena related to FFF and continue to develop new techniques and modifications of FFF. In the second category, the investigator will intensively develop flow FFF, the most widely applicable of FFF techniques. He will characterize and develop ultrathin channels for high- speed flow FFF analysis and undertake other studies to improve and automate this technique. In the third category, the investigator will utilize his on- line sample concentration technique to implement the coupling of sedimentation FFF to flow FFF for the characterization of complex colloidal fluids such as cell lysates. In the fourth category, he will develop applications and new application strategies. These will complement his basic studies. Applications work will be directed at biological cells, motile bacteria, protein complexes, protein inclusion bodies, colloids in blood plasma, liposomes, and other classes of biological and biomedical materials.

描述：拟议工作的主要目标是发展

项目成果

Simplified nonequilibrium theory of secondary relaxation effects in programmed field-flow fractionation.

程序化场流分级中二次弛豫效应的简化非平衡理论。

• DOI: 10.1021/ac00295a018
• 发表时间: 1986
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Giddings,JC

Origin and characterization of departures from the statistical model of component-peak overlap in chromatography.

色谱中组分峰重叠统计模型偏差的起源和表征。

• DOI: 10.1016/s0021-9673(00)95095-7
• 发表时间: 1984
• 期刊: Journal of chromatography
• 作者: Davis,JM;Giddings,JC
• 通讯作者: Giddings,JC

Dual-field and flow-programmed lift hyperlayer field-flow fractionation.

双场和程序流升力超层场流分级。

• DOI: 10.1021/ac00025a003
• 发表时间: 1992
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Ratanathanawongs,SK;Giddings,JC
• 通讯作者: Giddings,JC

Particle size distribution by sedimentation/steric field-flow fractionation: development of a calibration procedure based on density compensation.

通过沉降/空间场流分级进行粒度分布：开发基于密度补偿的校准程序。

• DOI: 10.1021/ac00014a006
• 发表时间: 1991
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Giddings,JC;Moon,MH;Williams,PS;Myers,MN
• 通讯作者: Myers,MN

Improved flow field-flow fractionation system applied to water-soluble polymers: programming, outlet stream splitting, and flow optimization.

改进的流场流分馏系统应用于水溶性聚合物：编程、出口流分裂和流量优化。

• DOI: 10.1021/ac00294a018
• 发表时间: 1986
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Wahlund,KG;Winegarner,HS;Caldwell,KD;Giddings,JC
• 通讯作者: Giddings,JC