Building long-read high-fidelity sequencing resources to support Bioscience Research in the UK

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The genomics revolution is entering a new phase, with new sequencing technologies that can read long DNA molecules with unprecedented accuracy and low costs. These high fidelity long DNA sequence reads can be used to generate high-quality gapless reference genomes, to fully characterise transcriptomes, to describe methylomes, and to characterize microorganisms in complex microbial mixtures such as in the gut microbiome or in environmental samples of soil or water. The PacBio Revio System is the latest long-read DNA sequencing platform that can generate extremely large amounts of long read data at an affordable cost. A key feature of the Revio is that it can generate highly accurate long reads, by reading a DNA molecule multiple times to correct errors. This grant will acquire the Revio platform, the first in Scotland, embed this within Edinburgh Genomics, and allow us to offer low-cost, high quality long read sequencing in collaboration with researchers in the biological and biotechnology research community. We will use the investment to support researchers who want to generate genomic data for diverse topics such as agriculture, biotechnology, genetics, immunology and synthetic biology. We will also offer advanced genomics data training to support the uptake of the Revio within the wider user community.