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BIOCHEMICAL APPROACHES TO THE CELL DIVISION PROBLEM

细胞分裂问题的生物化学方法

基本信息

批准号：
2180580
负责人：
JAMES T PARK
金额：
$ 2.81万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-07-01 至 1994-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2180580
关键词：
Escherichia coli aminoacyltransferase bacterial genetics bacterial proteins binding proteins cell cycle crosslink cytogenetics developmental genetics enzyme complex enzyme mechanism galactosyltransferases gene expression gram negative bacteria high performance liquid chromatography protein biosynthesis synchronous cell division temperature sensitive mutant

项目摘要

The long term objective of this research is to elucidate the biochemical steps required for septation and cell separation in a gram negative organism such as Escherichia coli. More specifically, the work will be concerned with identifying and characterizing the function of the proteins involved in formation and separation of new polar caps in the septum. Presumably a number of enzymes are needed for de novo synthesis of the polar caps but only one penicillin-binding protein 3, has been identified. Yet at least another twelve gene products are essential for septation. The research seeks to identify the role of these essential proteins. The specific aims are to: 1. Identify the chemical structures and enzymatic reactions that are unique to septation. and 2. Characterize multiprotein complexes involved in septation. The latter objective involves isolation and characterization of extragenic suppressors of cell division mutants, isolation of protein complexes by appropriate physical methods, and identification of the enzymatic activities and protein components therein. Obviously only a pioneering start can be made toward achieving the final objectives but understanding cell division is of such fundamental importance that it seems appropriate that someone begins a more direct attack to learn something about the role of the twelve or more gene products required for septation.

这项研究的长期目标是阐明分离和细胞分离所需的生化步骤革兰氏阴性生物，如大肠杆菌。更多具体地说，这项工作将涉及识别和表征参与形成的蛋白质的功能隔膜中新的极帽的分离。想必是一个极性的从头合成需要许多酶。但只有一种青霉素结合蛋白3已被鉴定。然而，至少还有另外12种基因产品是分隔符。这项研究试图确定这些因素的作用必需蛋白质。具体目标是：1.鉴定化学结构和隔膜所特有的酶反应。和2. 表征参与隔膜的多蛋白复合体。这个后一个目标涉及分离和鉴定细胞分裂突变体的基因外抑制物的分离通过适当的物理方法合成蛋白质复合体，以及酶活性和蛋白质组分的鉴定在那里。显然，只有开拓性的开始才能朝着实现最终目标，但了解细胞分裂是如此根本的重要性，以至于它似乎适合有人开始更直接的攻击，以了解一些关于隔膜所需的12个或更多基因产物的作用。