BIOCHEMICAL APPROACHES TO THE CELL DIVISION PROBLEM

细胞分裂问题的生物化学方法

• 批准号: 3298660
• 负责人: JAMES T PARK
• 金额: $ 16.08万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3298660
• 关键词: Escherichia coli; aminoacyltransferase; bacterial genetics; bacterial proteins; binding proteins; cell cycle; crosslink; cytogenetics; developmental genetics; enzyme complex; enzyme mechanism; galactosyltransferases; gene expression; gram negative bacteria; high performance liquid chromatography; protein biosynthesis; synchronous cell division; temperature sensitive mutant

The long term objective of this research is to elucidate the biochemical steps required for septation and cell separation in a gram negative organism such as Escherichia coli. More specifically, the work will be concerned with identifying and characterizing the function of the proteins involved in formation and separation of new polar caps in the septum. Presumably a number of enzymes are needed for de novo synthesis of the polar caps but only one penicillin-binding protein 3, has been identified. Yet at least another twelve gene products are essential for septation. The research seeks to identify the role of these essential proteins. The specific aims are to: 1. Identify the chemical structures and enzymatic reactions that are unique to septation. and 2. Characterize multiprotein complexes involved in septation. The latter objective involves isolation and characterization of extragenic suppressors of cell division mutants, isolation of protein complexes by appropriate physical methods, and identification of the enzymatic activities and protein components therein. Obviously only a pioneering start can be made toward achieving the final objectives but understanding cell division is of such fundamental importance that it seems appropriate that someone begins a more direct attack to learn something about the role of the twelve or more gene products required for septation.

这项研究的长期目标是阐明 在一个实施例中，分离和细胞分离所需的生物化学步骤 革兰氏阴性微生物如大肠杆菌。 更 具体而言，这项工作将涉及确定和 表征参与形成的蛋白质的功能 以及隔膜中新的极帽的分离。 可能是一 需要许多酶来从头合成极性的 caps，但只有一个青霉素结合蛋白3，已被确定。 然而，至少还有12种基因产物是必不可少的， 分隔 该研究旨在确定这些因素的作用， 必需蛋白质 具体目标是：1.识别化学结构， 酶促反应是分隔所特有的。 和2. 鉴定参与分隔的多蛋白复合物。 的 后一个目标涉及分离和表征 细胞分裂突变体的基因外抑制子的分离 通过适当的物理方法制备蛋白质复合物， 酶活性和蛋白组分的鉴定 在其中 显然，只有开创性的开始， 但理解细胞分裂是 如此重要的根本性， 有人开始了更直接的攻击， 分隔所需的12种或更多基因产物的作用。