GENE MAPPING AND GENOME ORGANIZATION IN MAMMALS

特异染色体基因标记的精确定位 染色体片段和变化的定量分析 需要在哺乳动物进化过程中定位这些标记 来描述哺乳动物进化的组织结构 来描述哺乳动物基因组的结构。 小鼠将被用作分析基因定位的模型， 阐明特定染色体片段上的基因顺序，以及 表征同线性和连锁保守的模式 在哺乳动物进化过程中。 这些研究需要汇编， 小鼠连锁数据和哺乳动物基因同源性数据的分析。 目前正在开发两种计算机资源，一旦完成， 将包括（1）原始小鼠基因图谱数据和分析 程序（MLDP，鼠标连接数据库和程序），和（2） 哺乳动物基因同源性数据和分析程序（HMDP，同源性 数据库和程序）。 MLDP和HMDP都将得到维护， 增强为拟议分析的资源和研究工具 和科学界（目标1）。 小鼠基因定位数据 MLDP中的分析程序将用于计算和 比较重组频率和图谱距离， 替代基因顺序的可能性， 代表遗传上明确定义的染色体片段的图谱 (Aim 2）。 小鼠连锁测试杂交将用于解决 对于在小鼠中位置不确定的基因， 以及小鼠中潜在保守的基因 哺乳动物的染色体片段（Aim 3）。 哺乳动物同线性和 将使用HMDP中的连锁同源性数据和分析程序 用于估计个体中同线保守的程度 哺乳动物谱系，并用于测试同线率是否 破坏在谱系之间差异显著（目标4）。 这些 拟议的研究代表了一个全面的计划， 小鼠基因定位数据和哺乳动物基因同源性数据， 利用这些数据来研究基因组的组织和进化， 哺乳动物

Accurate localization of gene markers for specific chromosomal segments and quantitative analyses of changes in chromosomal localization of these markers during mammalian evolution are needed to characterize the organization of the mammalian evolution are needed to characterize the organization of the mammalian genome. The mouse will be used as a model for analyzing gene location, clarifying gene order on specific chromosomal segments, and characterizing the patterns of synteny and linkage conservation during mammalian evolution. These studies require compilation and analysis of mouse linkage data and mammalian gene homology data. Two computer resources are being developed and that, when complete, will consist of (1) raw mouse gene mapping data and analysis programs (MLDP, mouse linkage database and programs), and (2) mammalian gene homology data and analysis programs (HMDP, homology database and programs). Both MLDP and HMDP will be maintained and enhanced as a resource and research tool for the proposed analyses and for the scientific community (Aim 1). Mouse gene mapping data and analytical programs in MLDP will be used for calculating and comparing recombination frequencies and map distances, determining the likelihood for alternative gene orders, and creating segmental maps that represent genetically well-defined chromosomal segments (Aim 2). Mouse linkage testing crosses will be used for resolving gene order both for genes whose location is uncertain in the mouse and for genes in the mouse that are part of potentially conserved chromosomal segments among mammals (Aim 3). Mammalian synteny and linkage homology data and analytical programs in HMDP will be used for estimating the extent of synteny conservation in individual mammalian lineages and for testing whether rates of synteny disruption vary significantly among lineages (Aim 4). These proposed studies represent a comprehensive program for managing mouse gene mapping data and mammalian gene homology data and for using these data to study genome organization and evolution in mammals.