SPATIAL PATTERN OF THE EGG CHAMBER IN DROSOPHILA

这个项目的目的是分析空间不对称如何 产生于果蝇发育中的卵室。 四个基因 （尖顶，卡布奇诺，gurken和鱼雷），这是必需的， 蛋壳和蛋壳的正确空间图案 将对胚胎进行分析。 其中三个基因是细胞所必需的 鱼雷在体细胞中发挥作用。 的 因此，分析集中在卵子发生的一个阶段， 蛋壳的形状和胚胎的形状 依赖于不同卵巢细胞之间的相互作用 类型 更精确地定义这四个基因在种系中的作用 和体细胞，新的等位基因和染色体重排 将分离在基因座处的野生型和 突变卵室将在整个坐骑中进行研究， 切片材料。 镶嵌卵室的分析 鱼雷，以及显性和隐性标记突变，将 用于研究卵泡细胞在卵泡发育中的作用， 卵室的图案。 一个初步的分子分析， 塔尖和鱼雷将被执行。 这将包括一个 基因组步移到含有两个基因座的染色体区域， 基因的鉴定，以及空间和 产品的时间分布。 这将使我们能够 发展一个分子模型的模式机制， 卵室

The aim of this project is the analysis of how spatial asymmetries arise in the developing egg chamber of Drosophila. Four genes (spire, cappuccino, gurken, and torpedo) which are required for the correct spatial patterning of both the egg shell and the embryo will be analyzed. Three of the genes are required in cells of the germline, wheras torpedo functions in somatic cells. The analysis focusses therefore on a stage in oogenesis when both the pattern of the egg shell and the pattern of the embryo are dependent on interactions between the different ovarian cell types. To define more precisely the role of the four genes in germline and somatic cells, new alleles and chromosomal rearrangements at the loci will be isolated, and the morphology of wildtype and mutant egg chambers will be studied in whole mounts and sectioned material. Analysis of mosaic egg chambers using torpedo, as well as dominant and recessive marker mutations, will be used to investigate the role of the follicle cells in the patterning of the egg chamber. An initial molecular analysis of spire and torpedo will be performed. This will consist of a genomic walk to the chromosomal region containing the two loci, identification of the genes, and an analysis of the spatial and temporal distribution of their products. This will allow us to develop a molecular model of patterning mechanisms acting in the egg chamber.