TRNA SPLICING ENDONUCLEASE IN YEAST
酵母中的 TRNA 剪接核酸内切酶
基本信息
- 批准号:3297722
- 负责人:
- 金额:$ 13.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-09-01 至 1993-08-31
- 项目状态:已结题
- 来源:
- 关键词:Saccharomyces alleles chemical binding endonuclease enzyme mechanism enzyme structure fungal genetics gene complementation gene expression gene interaction gene mutation genetic manipulation immunoelectron microscopy ligase microscopy molecular cloning molecular genetics nucleic acid structure radioimmunoassay structural genes transfer RNA
项目摘要
Splicing of intervening sequences from precursor-tRNA requires
at least two functions, an endonuclease and a ligase. These
enzymes catalyze excision of the introns and rejoining of the
tRNA half-molecules through recognition of conserved features of
tRNA structure. Despite the fact that tRNA splicing is readily
amenable to genetic and biochemical analysis, little is known
about the regulatory and structural genes involved, the potential
interaction of proteins or other components that might form a
splicing complex, or the possible relationship between splicing and
transport of tRNA from nucleus to cytoplasm. Information on
these aspects of tRNA splicing have not been forthcoming due to
the intractable nature of the membrane-associated tRNA-splicing
endonuclease. Previous attempts in several laboratories to study
the endonuclease by either forward or reverse genetic techniques
have not been successful. Recently, however, mutations in SEN
(splicing endonuclease) genes were identified in this laboratory
that cause partial deficiencies of endonuclease activity in the
yeast Saccharomyces cerevisiae. One mutant also causes pre-
tRNA steady-state accumulation and temperature-sensitive
growth. By analyzing SEN genes and their products using genetic,
biochemical, and immunological approaches the research in this
proposal will provide critical information on the structure,
organization, function, and intracellular location of tRNA splicing
components. The most immediate question to be answered
centers on which products are structural components of the
endonuclease. Once this central question is answered it should be
possible to address the more complex issues of how and where
tRNA splicing occurs in intact cells.
从Btensor-tRNA剪接插入序列需要
至少两种功能,核酸内切酶和连接酶。 这些
酶催化内含子的切除和内含子的重新连接
tRNA半分子通过识别的保守特征,
tRNA结构 尽管事实上tRNA剪接很容易
我们对基因和生化分析的结果知之甚少
关于相关的调节和结构基因,
蛋白质或其他成分的相互作用,
剪接复合体,或剪接和
tRNA从细胞核到细胞质的转运。 信息
tRNA剪接的这些方面还没有出现,
膜相关tRNA剪接的难处理性
核酸内切酶。 之前几个实验室尝试研究
通过正向或反向遗传技术
都没有成功。 然而,最近,SEN中的突变
(剪接核酸内切酶)基因在本实验室鉴定
其导致在细胞中核酸内切酶活性的部分缺陷,
酿酒酵母 一个突变体也会导致前-
tRNA稳态积累和温度敏感性
增长 通过使用遗传学方法分析SEN基因及其产物,
生物化学和免疫学的研究方法,
建议书将提供有关结构的重要信息,
tRNA剪接的组织、功能和细胞内定位
件. 最迫切需要回答的问题是
中心,产品是
核酸内切酶。 一旦这个核心问题得到回答,
如何以及在哪里解决更复杂的问题
tRNA剪接发生在完整的细胞中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL R CULBERTSON其他文献
MICHAEL R CULBERTSON的其他文献
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{{ truncateString('MICHAEL R CULBERTSON', 18)}}的其他基金
The NMD pathway in RNA surveillance and gene expression
RNA 监测和基因表达中的 NMD 通路
- 批准号:
6622911 - 财政年份:2002
- 资助金额:
$ 13.9万 - 项目类别:
The NMD pathway in RNA surveillance and gene expression
RNA 监测和基因表达中的 NMD 通路
- 批准号:
6732678 - 财政年份:2002
- 资助金额:
$ 13.9万 - 项目类别:
The NMD pathway in RNA surveillance and gene expression
RNA 监测和基因表达中的 NMD 通路
- 批准号:
6863721 - 财政年份:2002
- 资助金额:
$ 13.9万 - 项目类别:
The NMD pathway in RNA surveillance and gene expression
RNA 监测和基因表达中的 NMD 通路
- 批准号:
6459155 - 财政年份:2002
- 资助金额:
$ 13.9万 - 项目类别:
IMPROVING GENETICS/BIOTECHNOLOGY CENTER ANIMAL FACILITY
改善遗传学/生物技术中心动物设施
- 批准号:
6365710 - 财政年份:2001
- 资助金额:
$ 13.9万 - 项目类别:
FACTORS IN MATURATION OF SMALL RNAS IN THE YEAST NUCLEUS
酵母细胞核中小 RNA 成熟的因素
- 批准号:
6525509 - 财政年份:2000
- 资助金额:
$ 13.9万 - 项目类别:
FACTORS IN MATURATION OF SMALL RNAS IN THE YEAST NUCLEUS
酵母细胞核中小 RNA 成熟的因素
- 批准号:
6197443 - 财政年份:2000
- 资助金额:
$ 13.9万 - 项目类别:
FACTORS IN MATURATION OF SMALL RNAS IN THE YEAST NUCLEUS
酵母细胞核中小 RNA 成熟的因素
- 批准号:
6402654 - 财政年份:2000
- 资助金额:
$ 13.9万 - 项目类别:
FACTORS IN MATURATION OF SMALL RNAS IN THE YEAST NUCLEUS
酵母细胞核中小 RNA 成熟的因素
- 批准号:
6644826 - 财政年份:2000
- 资助金额:
$ 13.9万 - 项目类别:
GENETIC ANALYSIS OF TRNA SPLICING IN THE YEAST NUCLEUS
酵母细胞核中 TRNA 剪接的遗传分析
- 批准号:
3297723 - 财政年份:1988
- 资助金额:
$ 13.9万 - 项目类别:
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