BRAIN MECHANISMS OF REPRODUCTIVE BEHAVIOR IN RATS

大鼠生殖行为的大脑机制

• 批准号: 3310419
• 负责人: Donald W. Pfaff
• 金额: $ 21.84万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310419
• 关键词: autoradiography; brain stem; complementary DNA; denervation; electromyography; electron microscopy; electrophysiology; electrostimulus; estrogens; experimental brain lesion; female; gene expression; histochemistry /cytochemistry; hormone regulation /control mechanism; hypothalamus; immunochemistry; mesencephalon; messenger RNA; microinjections; neural transmission; neuroendocrine system; neuropsychology; progesterone; psychobiology; radiotracer; reticular formation; reticulospinal tract; ribosomal RNA; sex behavior; sex hormones; single cell analysis; spinal cord mapping

Mechanisms of female reproductive behavior, especially regarding hormone effects on the lordosis response, are among the best worked out in mammalian brain. Knowledge of its neural circuitry provides a platform for looking with increased detail at cellular mechanisms for this behavior, which is required for reproduction because it allows fertilization by the male. Following extensive analyses of cellular changes following estradiol (E), it now is easier to study cellular changes which underline progesterone (p)-facilitated behavior. Because effects of progesterone on lordosis require new protein synthesis, classical progestin receptors may play a role. We will use steroid autoradiography to look at receptor/behavior correlations as a function of estrogen dose and anti-progestin dose. What proteins do behaviorally important progestin-receiving cells produce? We will use our technique of combining immunocytochemistry with steroid autoradiography to colocalize nuclear progestin receptors and each of several behaviorally relevant neural proteins. Where do progesterone-receiving cells send their axons? We will combine retrograde neuroanatomical techniques with steroid autoradiography in the same tissue to see if progestin-sensitive cells send axons to certain terminal zones, each implicated in lordosis behavior. In vitro single unit electrophysiology is a chemically clean method of investigating hormone effects on hypothalamic neurons. We will look for electrophysiological/behavioral correlations by giving different E or E+P doses in vivo, testing for lordosis, and studying ventromedial hypothalamic single unit responses to behaviorally active peptides and transmitters. Ultrastructural effects of hormones give clues to guide physiological and behavioral experiments. We will use electron microscopy to look at effects of estrogens and progestins on hypothalamic neurons under conditions which allow morphological/behavioral correlations across hormone treatment durations or amounts of receptor site occupation. Hypothalamic neuron axonal terminals in other brain regions will also be examined. We have developed the application of in situ hybridization techniques to brain tissue, for using labeled cDNA to detect specific messenger RNA in individual cell groups. We will use this technique to study hormone effects on ribosomal RNA and messenger RNA levels for LHRH and proenkephalin.

女性生殖行为的机制，特别是与激素有关的机制 对脊柱前凸反应的影响是最好的研究之一 哺乳动物的大脑。 其神经回路的知识提供了一个平台 更详细地研究这种行为的细胞机制， 这是繁殖所必需的，因为它允许受精 男性。 经过对雌二醇 (E) 后细胞变化的广泛分析， 现在更容易研究强调黄体酮的细胞变化 (p)-促进行为。 因为黄体酮对脊柱前凸的影响 需要新的蛋白质合成，经典孕激素受体可能发挥作用 角色。 我们将使用类固醇放射自显影术来观察受体/行为 作为雌激素剂量和抗孕激素剂量的函数的相关性。 什么 孕激素接受细胞产生对行为重要的蛋白质吗？ 我们 将使用我们将免疫细胞化学与类固醇相结合的技术 放射自显影以共定位核孕激素受体和每个 几种行为相关的神经蛋白。 去哪里 黄体酮接收细胞发送轴突？ 我们将结合逆行 在同一组织中使用类固醇放射自显影的神经解剖技术 看看孕激素敏感细胞是否将轴突发送到某些终端区域， 每个都与脊柱前凸行为有关。 体外单单元电生理学是一种化学清洁方法 研究激素对下丘脑神经元的影响。 我们会寻找 通过给出不同的 E 或 E+P 来确定电生理/行为相关性 体内剂量、脊柱前凸测试以及下丘脑腹内侧研究 对行为活性肽和递质的单一单位反应。 激素的超微结构效应为指导生理和心理提供了线索 行为实验。 我们将使用电子显微镜来观察效果 在以下条件下雌激素和孕激素对下丘脑神经元的影响 允许激素治疗过程中的形态/行为相关性 受体位点占据的持续时间或数量。 下丘脑神经元 其他大脑区域的轴突末梢也将受到检查。 我们开发了原位杂交技术的应用 脑组织，用于使用标记的 cDNA 检测脑组织中的特定信使 RNA 单个细胞群。 我们将使用这种技术来研究激素 LHRH 和 LHRH 对核糖体 RNA 和信使 RNA 水平的影响 脑啡肽原。