RELAXIN--BIOCHEMICAL AND PHYSIOLOGICAL STUDIES

本应用程序的长期目标是更好地理解 妊娠大鼠松弛素分泌的调节及测定 松弛素对妊娠大鼠和猪的生理效应。 妊娠晚期产仔数对松弛素分泌的影响 将通过调整怀孕早期的受胎数来确定 并测定频繁采血中的松弛素浓度 是在产前收集的。母性的影响 妊娠后半期脑下垂体对松弛素分泌的影响 通过对怀孕大鼠(第13天)进行脑垂体切除来确定，其中包含 一个完整的概念或一个概念并确定 在第14、16、18天采集血液和卵巢中的松弛素浓度， 怀孕20%。内源性母体黄体生成素的影响 将测定妊娠晚期对松弛素分泌的激素(LH) 用去糖化的人黄体生成素拮抗剂掩蔽黄体生成素受体 绒毛膜促性腺激素和松弛素血浓度的测定 怀孕最后三天的外周血样。 松弛素在大鼠出生时的生理作用将被确定 通过观察剥夺内源性松弛素后的后果。这 将通过被动中和松弛素与大鼠抗体来完成 松弛素，也通过降低内源性松弛素水平通过去除 几乎所有的黄体库。松弛素对血管张力的影响 宫颈结缔组织的特性及生化组成 猪的怀孕将通过比较这些特征来确定。 在取自正常妊娠猪和去卵巢的猪的宫颈中 孕猪用黄体酮或黄体酮加松弛素处理。这个 新鲜采集的头骨和颈椎切片的颈椎蠕变速度 宫颈的尾部部分将被确定。生物化学 将描述的宫颈的组件是 糖胺多聚糖、蛋白多聚糖和胶原蛋白。

The long-term objectives of this application are to better understand the regulation of relaxin secretion in the pregnant rat and to determine the physiological effects of relaxin in both the pregnant rat and pig. The influence of litter size on relaxin secretion during late pregnancy will be determined by adjusting conceptus numbers during early pregnancy and determining relaxin concentrations in frequent blood samplings collected during the antepartum period. The influence of the maternal pituitary on relaxin secretion during the second half of pregnancy will be determined by hypophysectomizing pregnant rats (day 13) which contain either a full complement of conceptuses or one conceptus and determining relaxin concentrations in blood and ovaries collected on days 14, 16, 18, and 20 of pregnancy. The influence of endogenous maternal luteinizing hormone (LH) on relaxin secretion during late pregnancy will be determined by masking LH receptors with the LH antagonist deglycosylated human chorionic gonadotropin and determining relaxin concentrations in the peripheral blood during the last three days of pregnancy. The physiological effects of relaxin at birth in the rat will be determined by observing the consequences of deprivation of endogenous relaxin. This will be done by passive neutralization of relaxin with antibodies for rat relaxin and also by reduction of endogenous relaxin levels by removal of nearly all of the corpora lutea. The influence of relaxin on the tensile properties and biochemical composition of cervical connective tissue during pregnancy in the pig will be determined by comparing these characteristics among cervices obtained from intact pregnant pigs and ovariectomized pregnant pigs treated with progesterone or progesterone plus relaxin. The cervical creep rate of freshly collected sections of the cephalic and caudal portions of the cervix will be determined. The biochemical components of the cervix which will be characterized are those of the glycosaminoglycans, proteoglycans, and collagen.