RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
批准号:
2196626
负责人:
ORRIN David SHERWOOD
金额:
$12.37万
依托单位国家:
美国
项目类别:
财政年份:
1977
资助国家:
美国
项目状态:
已结题
起止时间:
1977-12-01 至 1997-07-31
关键词:
RNase protection assay birth cell biology cervix collagenase enzyme activity estrogens hormone receptor hormone regulation /control mechanism immunocytochemistry in situ hybridization laboratory mouse laboratory rat light microscopy messenger RNA physiology pregnancy prostaglandin endoperoxide synthase relaxin stromelysin transmission electron microscopy vagina western blottings
中文摘要
在人类和其他哺乳动物的怀孕后期
英文摘要
During late pregnancy in humans and other mammalian species, the
consistency of the cervix softens, thus enabling cervical dilatation and
rapid delivery of the fetus(es). In cases of difficult spontaneous
delivery or induced delivery at term, a hard inelastic cervix may be a
source of complications. The hormone relaxin is an effective cervical
softening agent with potential for clinical application in women and
domestic animals. Informed decisions concerning the use of relaxin as
a cervical softening agent will largely depend upon basic studies of its
effects in species other than the human. The principal long-range
objective of this application is to better understand at the cellular and
molecular levels the mechanism(s) whereby relaxin, in concert with
estrogen, promotes growth and softening of the cervix in pregnant rats.
To determine the influence of estrogen and relaxin on (1) the composition
and ultrastructure, as well as (2) the proliferation of cells within the
cervix, rats will be ovariectomized on day 9 of pregnancy and thereafter
receive hormone replacement therapy with physiological levels of
progesterone, progesterone and estrogen, or progesterone, estrogen, and
relaxin. Cervices will be collected late in pregnancy (day 22) and the
effects of estrogen and relaxin on the cervical cells will be determined
by light and electron microscopy. Immunohistochemistry will be used to
(3) identify the target cells for relaxin in the cervix. Ovariectomized
hormone-treated pregnant rats will also be used for studies aimed at
determining the influence of estrogen and relaxin on enzymes
(prostaglandin synthase, collagenase, stromelysin) synthesized by rat
cervices. The quantities of enzymes within cervical tissues will be
determined by Western blot analysis and the cellular localization of the
enzymes will be done by immunocytochemical analysis. The quantities of
mRNA for the cervical enzymes will be determined by ribonuclease
protection assay and the localization of the mRNA for enzymes will be
done by in situ hybridization. Specific Aim 5 of this application is to
determine if endogenous relaxin has physiological effects on the vagina
in pregnant rats. The consequences of neutralization of relaxin with a
monoclonal antibody for rat relaxin on the growth, tensile properties,
and histological characteristics of the vagina will be determined.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Fourth International Conf. on Relaxin & Related Peptides
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批准号:6669354
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项目类别:
-
资助金额:$0.6万
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财政年份:2004
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:6469880
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项目类别:
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资助金额:$22.91万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:6706306
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项目类别:
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资助金额:$23.26万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:6873021
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项目类别:
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资助金额:$23.76万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:6660223
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项目类别:
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资助金额:$1.08万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:7036484
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项目类别:
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资助金额:$23.2万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
Hormonal Regulation of the Cervix During Pregnancy
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批准号:6623700
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项目类别:
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资助金额:$25.78万
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财政年份:2002
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负责人:ORRIN David SHERWOOD
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依托单位:
IDENTIFICATION OF RELAXIN'S TARGET CELLS IN THE CERVIX
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批准号:3023141
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项目类别:
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资助金额:$3.33万
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财政年份:1989
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负责人:ORRIN David SHERWOOD
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依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310967
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项目类别:
-
资助金额:$10.48万
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财政年份:1977
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负责人:ORRIN David SHERWOOD
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依托单位:
RELAXIN--BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310964
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项目类别:
-
资助金额:$12.77万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:2196627
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项目类别:
-
资助金额:$13.1万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN--BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310966
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项目类别:
-
资助金额:$12.84万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310968
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项目类别:
-
资助金额:$9.51万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN--BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310960
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项目类别:
-
资助金额:$12.47万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310963
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项目类别:
-
资助金额:$11.38万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310969
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项目类别:
-
资助金额:$9.95万
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财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310961
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项目类别:
-
资助金额:$9.83万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
-
依托单位:
RELAXIN: BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:2196625
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项目类别:
-
资助金额:$11.81万
-
财政年份:1977
-
负责人:ORRIN David SHERWOOD
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依托单位:
RELAXIN--BIOCHEMICAL AND PHYSIOLOGICAL STUDIES
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批准号:3310965
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项目类别:
-
资助金额:$11.85万
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财政年份:1977
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负责人:ORRIN David SHERWOOD
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依托单位:
PROGRAM OF RESEARCH TRAINING IN REPRODUCTIVE BIOLOGY
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批准号:2838654
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项目类别:
-
资助金额:$12.14万
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财政年份:1976
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负责人:ORRIN David SHERWOOD
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依托单位:
海外基金