CATALYSIS OF THIOL/DISULFIDE EXCHANGE

硫醇/二硫化物交换的催化

• 批准号: 3297834
• 负责人: HIRAM F GILBERT
• 金额: $ 9.99万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297834
• 关键词: chemical reaction; disulfide bond; enzyme mechanism; glutathione; isomerase; oxidation reduction reaction; potentiometry; synthetic peptide; thermodynamics; thiols

Disulfide bond formation is an integral part of the expression of numerous extracellular proteins including receptors, enzymes, and peptide hormones. A fundamental understanding of disulfide bond formation will ultimately be essential to the design of any disulfide-bonded protein or peptide targeted to the extracellular environment by gene therapy expression systems. Shortly after translation on the endoplasmic reticulum, proteins containing disulfide bonds must correctly fold and form specific sets of disulfide bonds. This process is very rapid in vivo, and is likely to be catalyzed by an enzyme--protein disulfide isomerase. The long-term goal of this research is to understand the mechanism of correct disulfide bond formation, to describe the thermodynamic and kinetic constraints on the intracellular compartment where disulfide bond formation occurs, and to investigate mechanisms by which disulfide bond formation can be catalyzed. Simple predictions of the rate of oxidation and reduction of dithiols and disulfides suggest that the effectiveness of any catalyst for protein oxidation will depend on the relationship between the redox status of the environment and the oxidation potentials of the protein and any catalyst. Immediate experimental goals involve determining the efficiency of catalyzed and uncatalyzed protein oxidation and reduction as a function of the oxidation potential and redox status of the redox buffer. A series of hydrophilic peptides containing two cysteine residues located 1 to 9 residues apart will be synthesized to provide a spectrum of redox buffers of different oxidation potentials. These peptide redox buffers will be used to set the redox poise of the medium in which protein disulfide bond formation occurs. The formation and cleavage of disulfide bonds in several well-characterized proteins (bovine trypsin inhibitor, ribonuclease A, and lysozyme) will be studied in the presence and absence of protein disulfide isomerase in these dithiol/disulfide peptide redox buffers. The oxidation potential of protein disulfide isomerase will be determined. The achievement of these experimental objectives will lead to an enhanced understanding of the complex mechanisms by which protein disulfide bonds are formed during the expression of disulfide-bonded proteins and how these processes are catalyzed.

二硫键的形成是表达 许多细胞外蛋白质，包括受体、酶和 肽激素 对二硫键的基本认识 形成最终将是必不可少的任何设计 二硫键结合的蛋白质或肽靶向细胞外 环境基因治疗表达系统。 后不久 在内质网上翻译，蛋白质含有 二硫键必须正确折叠并形成特定的 二硫键 这一过程在体内非常迅速， 由一种酶催化蛋白质二硫键异构酶 这项研究的长期目标是了解 正确的二硫键形成机制，以描述 细胞内的热力学和动力学约束 发生二硫键形成的隔室，以及 研究二硫键形成机制， 催化的 简单预测氧化速率， 二硫醇和二硫化物的还原表明， 任何蛋白质氧化催化剂的活性都取决于 环境的氧化还原状态与 蛋白质和任何催化剂的氧化电位。 近期实验目标包括确定 催化和非催化的蛋白质氧化和还原， 氧化还原的氧化电位和氧化还原状态的函数 缓冲液 一系列含有两个半胱氨酸的亲水肽 位于相隔1至9个残基的残基将被合成， 提供了一系列不同氧化的氧化还原缓冲剂 潜力 这些肽氧化还原缓冲液将用于设定肽的氧化还原速率。 蛋白质二硫键在介质中的氧化还原平衡 形成发生。 二硫键的形成和断裂 在几种充分表征的蛋白质（牛胰蛋白酶抑制剂， 核糖核酸酶A和溶菌酶）将在存在和 在这些二硫醇/二硫化物中不存在蛋白质二硫键异构酶 肽氧化还原缓冲液。 蛋白质二硫键的氧化电位 将确定异构酶。 这些实验目标的实现将导致 加强对复杂机制的理解， 蛋白质二硫键是在表达 二硫键蛋白质以及这些过程是如何催化的。