BRAIN MECHANISMS OF REPRODUCTIVE BEHAVIOR

生殖行为的大脑机制

• 批准号: 3310416
• 负责人: Donald W. Pfaff
• 金额: $ 2.07万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310416
• 关键词: autoradiography; brain stem; complementary DNA; denervation; electromyography; electron microscopy; electrophysiology; electrostimulus; enkephalins; estrogen receptors; estrogens; experimental brain lesion; female; gene expression; histochemistry /cytochemistry; hormone regulation /control mechanism; hypothalamus; immunochemistry; immunocytochemistry; in situ hybridization; laboratory rat; mesencephalon; messenger RNA; microinjections; neural transmission; neuroendocrine system; neuropeptides; neuropsychology; progesterone; psychobiology; radiotracer; reticular formation; reticulospinal tract; ribosomal RNA; sex behavior; sex hormones; single cell analysis; spinal cord mapping

Mechanisms of female reproductive behavior, especially regarding hormone effects on the lordosis response, are among the best worked out in mammalian brain. Knowledge of its neural circuitry provides a platform for looking with increased detail at cellular mechanisms for this behavior, which is required for reproduction because it allows fertilization by the male. Following extensive analyses of cellular changes following estradiol (E), it now is easier to study cellular changes which underline progesterone (p)-facilitated behavior. Because effects of progesterone on lordosis require new protein synthesis, classical progestin receptors may play a role. We will use steroid autoradiography to look at receptor/behavior correlations as a function of estrogen dose and anti-progestin dose. What proteins do behaviorally important progestin-receiving cells produce? We will use our technique of combining immunocytochemistry with steroid autoradiography to colocalize nuclear progestin receptors and each of several behaviorally relevant neural proteins. Where do progesterone-receiving cells send their axons? We will combine retrograde neuroanatomical techniques with steroid autoradiography in the same tissue to see if progestin-sensitive cells send axons to certain terminal zones, each implicated in lordosis behavior. In vitro single unit electrophysiology is a chemically clean method of investigating hormone effects on hypothalamic neurons. We will look for electrophysiological/behavioral correlations by giving different E or E+P doses in vivo, testing for lordosis, and studying ventromedial hypothalamic single unit responses to behaviorally active peptides and transmitters. Ultrastructural effects of hormones give clues to guide physiological and behavioral experiments. We will use electron microscopy to look at effects of estrogens and progestins on hypothalamic neurons under conditions which allow morphological/behavioral correlations across hormone treatment durations or amounts of receptor site occupation. Hypothalamic neuron axonal terminals in other brain regions will also be examined. We have developed the application of in situ hybridization techniques to brain tissue, for using labeled cDNA to detect specific messenger RNA in individual cell groups. We will use this technique to study hormone effects on ribosomal RNA and messenger RNA levels for LHRH and proenkephalin.

女性生殖行为的机制，特别是关于激素的机制 对前凸反应的影响，是在 哺乳动物的大脑。对其神经回路的了解为 通过更详细地观察这种行为的细胞机制， 这是生殖所必需的，因为它允许通过 男性。 在对雌激素(E)后的细胞变化进行广泛的分析后， 现在更容易研究黄体酮的细胞变化。 (P)--促成行为。因为黄体酮对前凸的影响 需要新的蛋白质合成，经典的孕激素受体可能发挥作用 角色。我们将使用类固醇放射自显影来观察受体/行为 雌激素剂量和抗孕激素剂量之间的相关性。什么 行为上重要的孕激素受体细胞产生的蛋白质？我们 将使用我们将免疫细胞化学与类固醇相结合的技术 共定位核孕激素受体的放射自显影 几种与行为相关的神经蛋白。在哪里？ 黄体酮受体细胞发送轴突？我们将结合逆行 神经解剖学技术在同一组织中的类固醇放射自显影 为了了解孕激素敏感细胞是否将轴突发送到特定的终末区， 每一个都牵涉到前凸行为。 体外单单位电生理学是一种化学清洁的方法 研究激素对下丘脑神经元的影响。我们会寻找 给予不同E或E+P的电生理/行为相关 体内剂量，测试前凸，并研究下丘脑腹内侧 单个单位对行为活性多肽和递质的反应。 激素的超微结构效应为引导生理学和 行为实验。我们将使用电子显微镜来观察效果 雌激素和孕激素对下丘脑神经元的影响 允许跨激素治疗的形态/行为相关 受体位置占用的持续时间或数量。下丘脑神经元 其他脑区的轴突终末也将被检查。 我们开发了原位杂交技术的应用于 脑组织，用于用标记的cDNA检测脑组织中的特异性信使RNA 单个细胞群。我们将使用这项技术来研究荷尔蒙 LHRH和LHRH对核糖体RNA和信使RNA水平的影响 脑啡肽原。