CARBOHYDRATE ENERGY ABSORPTION IN PREMATURE INFANTS

早产儿的碳水化合物能量吸收

• 批准号: 3317306
• 负责人: Craig Lawrence Kien
• 金额: $ 3万
• 依托单位: CHILDREN'S HOSPITAL (COLUMBUS)
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3317306
• 关键词: baby food; bioenergetics; breath composition; carbohydrate metabolism; colon; developmental nutrition; dietary carbohydrates; enteric bacteria; fatty acid metabolism; feces analysis; female; gastrointestinal nutrient absorption; gestational age; human milk; human subject; hydrogen; lactose; nutrition related tag; premature infant human

Previous studies of carbohydrate fermentation in the premature infant have shown: (1) High breath H2 concentrations indicative of lactose fermentation in the colon; (2) A low rate of fecal excretion of carbohydrate-derived carbon implying colonic salvage of fermentation products such as lactate and short-chain fatty acids (SCFA) (e.g., acetate). These data have relevance to the following questions involving nutrition of the premature infant: (1) Energy balance and how it is affected by antibiotic therapy and fermentation pathways. (2) Inter-relationships of post- conceptional age, lactose intake and fermentation pathways (level of H2 production; relative production of acetate versus lactate). (3) The relationship of colonic SCFA production to energy metabolism. The experimental subjects for this study will be premature infants, (28-32 wk gestational age and 1-4 wk post-natal age) fed a premature infant formula (carbohydrate: 100% lactose). The Specific Aims of this proposal are the following: (1) Experiment 1: To compare two methods of assessing fecal carbohydrate energy (carbon) excretion based respectively on the partition of fecal energy losses; fecal excretion of excess 13C after a single dose of (D-1-13C)-lactose. (2) Experiment 2: In weekly studies beginning at 1 wk of age, to assess the inter- relationships of gestational and post-natal age, lactose intake, breath H2 excretion, 13C enrichment of fecal acetate and lactate, and the rate of fecal excretion of SCFA'S and of the 13C derived from lactose. (3) Experiment 3: To determine whether short-term (5-7 d) broad spectrum antibiotic therapy and parenteral nutrition affects the inter-relationships in Experiment 2. (4) Experiment 4: Using a repeated, enteral-dose, (1-13C)-acetate, turnover technique, to assess the rate of total body acetate production and oxidation in subjects participating in Experiment 2 (1-2 wk of age and again at 3-4 wk). Ten infants with existing arterial lines and 10 young adult volunteers receiving lactulose will serve as controls to assess the methodology. Our Secondary Specific Aims include: (1) To assess carbon dioxide production and the efficiency of energy utilization in relation to factors studied in Experiments 2 and 4, particularly the relative 13C enrichments of lactate and acetate (after 13C-lactose) and their rates of fecal excretion. (2) To assess the small intestinal absorption of lactose by monitoring the 13C enrichment of breath CO2.

先前对早产的碳水化合物发酵的研究 婴儿已经显示出：（1）高呼吸H2浓度指示 结肠中的乳糖发酵； （2）低粪便率 碳水化合物衍生的碳的排泄意味着结肠打捞 发酵产品（例如乳酸和短链脂肪） 酸（SCFA）（例如乙酸）。 这些数据与 以下涉及早产营养的问题： （1）能量平衡及其如何受抗生素疗法的影响和 发酵途径。 （2）后的关系 构思年龄，乳糖摄入和发酵途径（水平 H2生产；乙酸与乳酸的相对产生）。 （3）结肠SCFA生产与能量的关系 代谢。 这项研究的实验学科将是 早产儿（28-32周胎龄和1-4周产后 年龄）喂养早产婴儿配方奶（碳水化合物：100％乳糖）。 该提案的具体目的是以下：（1） 实验1：比较两种评估粪便的方法 分别基于碳水化合物能量（碳）排泄 粪便能量损失的分区；超过13c的粪便排泄 单剂量（D-1-13C） - 乳糖之后。 （2）实验2：在 每周从1周开始的研究，以评估 妊娠和产后年龄，乳糖摄入量的关系， 呼吸H2排泄，13c乙酸粪便和乳酸的富集， SCFA和13C的粪便排泄速率 来自乳糖。 （3）实验3：确定是否短期 （5-7 d）广谱抗生素疗法和肠胃外营养 影响实验2中的相互关系。（4）实验 4：使用重复的肠剂量，（1-13c） - 乙酸盐，营业额 技术，以评估体内乙酸盐产量的速率和 参加实验2的受试者的氧化（1-2周龄 再次在3-4周）。 十个具有现有动脉线的婴儿 10名接受乳乳糖的年轻成人志愿者将作为 控制方法。 我们的次要特定目标 包括：（1）评估二氧化碳的产生和 与研究因素有关的能源利用效率 实验2和4，尤其是相对13C的富集 乳酸和乙酸酯（13c乳糖之后）及其粪便速率 排泄。 （2）评估小肠吸收 乳糖通过监测呼吸二氧化碳的13C富集。