BRAIN MECHANISMS OF REPRODUCTIVE BEHAVIOR

生殖行为的大脑机制

• 批准号: 3310415
• 负责人: Donald W. Pfaff
• 金额: $ 24.6万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310415
• 关键词: alpha adrenergic agent; alpha antiadrenergic agent; beta antiadrenergic agent; electrophysiology; endorphins; enkephalins; estradiol; gene expression; histochemistry /cytochemistry; hormone regulation /control mechanism; hypothalamus; immunocytochemistry; in situ hybridization; laboratory rat; muscarinic receptor; neural transmission; neuroendocrine system; neurons; neuropeptides; neurophysiology; neurotransmitters; nucleic acid hybridization; oxytocin; preoptic areas; progesterone; sex behavior

Lordosis, as part of female rat reproduction, provides a unique opportunity to relate molecular changes to behavior, because of the great amount of previous work on its hormonal mechanisms, sensory and motor components, and circuitry. Several neurochemical products have been implicated in lordosis control. We have selected strong candidates for relating molecular synthesis of specific transmitters and peptides to lordosis. We suspect that different gene expression systems may play different roles in the neuro-endocrine control of reproduction, and need to explore how each is related to lordosis. Thus, we will use in situ hybridization and single unit activity to compare molecular and electrophysiological results to lordosis measures. In turn, to tie these cellular results to behavior, where possible, we will manipulate molecular events to cause lordosis changes. Experiment set I. deals with 2 transmitter systems involved in lordosis in the rat hypothalamus: alpha-1 adrenergic and muscarinic. For each we will study the ability of E and P to alter messenger RNA levels for the appropriate receptor, and will use in vitro recordings of single unit activity to study E and P effects on hypothalamic neuronal responses to the appropriate agonists. Results with these two cellular approaches will be related to the effects of alpha-1 adrenergic agonists and muscarinic agonists on lordosis. Where possible, situational constraints on the actions of a given system will be hypothesized and tested. Experiment set II. deals with 3 neuropeptide systems involved in lordosis. LHRH gene expression will be studied under E and P conditions designed to allow tight correlations with lordosis, and its electrophysiological effects as a neuromodulator will be analyzed. Enkephalin gene expression will be correlated with lordosis performance under selected hormonal conditions. Oxytocin gene expression will be studied as a function of situational constraints which may reveal the nature of oxytocin's contributions to lordosis. Throughout, identification of the neuronal groups studied will be confirmed using combinations of techniques: we will follow electrophysiological and behavioral measurements with histochemical examination of the same tissue, including in situ hybridization and immunocytochemistry. Thus, we will be better able to see how molecular events in particular neuronal groups contribute to the occurrence of lordosis. Overall, the design of this project and the technical history of this lab allow detailed approaches to reductionistic brain mechanisms while maintaining a strict behavioral focus.

脊柱前凸，作为雌性大鼠生殖的一部分， 将分子变化与行为联系起来，因为大量的 以前的工作，其激素机制，感觉和运动组件， 电路 几种神经化学产物与脊柱前凸有关 控制 我们已经选择了强有力的候选人， 合成特定的递质和肽来调节脊柱前凸。 我们怀疑 不同的基因表达系统可能在基因表达中扮演不同的角色。 生殖的神经内分泌控制，并需要探讨如何每一个是 与脊柱前凸有关。 因此，我们将使用原位杂交和单个 单位活动，以比较分子和电生理结果， 脊柱前凸测量。 反过来，为了将这些细胞结果与行为联系起来， 在可能的情况下，我们将操纵分子事件来引起脊柱前凸 变化 实验组I。 涉及2个变送器系统， 大鼠下丘脑的脊柱前凸：α-1肾上腺素能和毒蕈碱。 为 我们将研究E和P改变信使RNA水平的能力， 合适的受体，并将使用单个单位的体外记录 活性，以研究E和P对下丘脑神经元对 合适的激动剂。 这两种细胞方法的结果将是 与α-1肾上腺素能激动剂和毒蕈碱的作用有关 激动剂的作用 在可能的情况下， 将假设和测试给定系统的动作。 实验台 二.涉及脊柱前凸的3种神经肽系统。 LHRH基因 表达式将在E和P条件下进行研究， 与脊柱前凸的相关性，以及其电生理效应， 将分析神经调节剂。 脑啡肽基因的表达 与选定激素条件下的脊柱前凸表现相关。 催产素基因的表达将被研究作为一个功能的情景 限制，这可能揭示催产素的贡献， 脊柱前凸 在整个过程中，识别所研究的神经元组将 使用技术组合进行确认：我们将遵循 电生理学和行为学测量与组织化学 检查相同的组织，包括原位杂交， 免疫细胞化学 因此，我们将能够更好地看到分子 特别是神经元组中的事件有助于发生 脊柱前凸 总的来说，该项目的设计和技术历史， 这个实验室允许详细的方法来简化大脑机制， 保持严格的行为专注。