BRAIN MECHANISMS OF REPRODUCTIVE BEHAVIOR

生殖行为的大脑机制

• 批准号: 3310420
• 负责人: Donald W. Pfaff
• 金额: $ 23.37万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310420
• 关键词: autoradiography; brain stem; complementary DNA; denervation; electromyography; electron microscopy; electrophysiology; electrostimulus; enkephalins; estrogen receptors; estrogens; experimental brain lesion; female; gene expression; histochemistry /cytochemistry; hormone regulation /control mechanism; hypothalamus; immunochemistry; immunocytochemistry; in situ hybridization; laboratory rat; mesencephalon; messenger RNA; microinjections; neural transmission; neuroendocrine system; neuropeptides; neuropsychology; progesterone; psychobiology; radiotracer; reticular formation; reticulospinal tract; ribosomal RNA; sex behavior; sex hormones; single cell analysis; spinal cord mapping

Mechanisms of female reproductive behavior, especially regarding hormone effects on the lordosis response, are among the best worked out in mammalian brain. Knowledge of its neural circuitry provides a platform for looking with increased detail at cellular mechanisms for this behavior, which is required for reproduction because it allows fertilization by the male. Following extensive analyses of cellular changes following estradiol (E), it now is easier to study cellular changes which underline progesterone (p)-facilitated behavior. Because effects of progesterone on lordosis require new protein synthesis, classical progestin receptors may play a role. We will use steroid autoradiography to look at receptor/behavior correlations as a function of estrogen dose and anti-progestin dose. What proteins do behaviorally important progestin-receiving cells produce? We will use our technique of combining immunocytochemistry with steroid autoradiography to colocalize nuclear progestin receptors and each of several behaviorally relevant neural proteins. Where do progesterone-receiving cells send their axons? We will combine retrograde neuroanatomical techniques with steroid autoradiography in the same tissue to see if progestin-sensitive cells send axons to certain terminal zones, each implicated in lordosis behavior. In vitro single unit electrophysiology is a chemically clean method of investigating hormone effects on hypothalamic neurons. We will look for electrophysiological/behavioral correlations by giving different E or E+P doses in vivo, testing for lordosis, and studying ventromedial hypothalamic single unit responses to behaviorally active peptides and transmitters. Ultrastructural effects of hormones give clues to guide physiological and behavioral experiments. We will use electron microscopy to look at effects of estrogens and progestins on hypothalamic neurons under conditions which allow morphological/behavioral correlations across hormone treatment durations or amounts of receptor site occupation. Hypothalamic neuron axonal terminals in other brain regions will also be examined. We have developed the application of in situ hybridization techniques to brain tissue, for using labeled cDNA to detect specific messenger RNA in individual cell groups. We will use this technique to study hormone effects on ribosomal RNA and messenger RNA levels for LHRH and proenkephalin.

女性生殖行为的机制，特别是关于激素 对脊柱前凸反应的影响，是最好的研究之一， 哺乳动物的大脑 对它的神经回路的了解提供了一个平台， 通过观察这种行为的细胞机制， 这是繁殖所必需的，因为它允许受精， 男性 在广泛分析雌二醇（E）后的细胞变化后， 现在更容易研究细胞变化， （p）促进行为。 因为黄体酮对脊柱前凸的影响 需要新的蛋白质合成，经典的β受体可能发挥作用， 作用 我们将使用类固醇放射自显影来观察受体/行为 相关性作为雌激素剂量和抗雌激素剂量的函数。 什么 孕激素接受细胞产生的重要行为蛋白质？ 我们 将使用我们的免疫细胞化学和类固醇相结合的技术 放射自显影术以共定位核内的白细胞介素受体， 几种与行为相关的神经蛋白。 的那里 孕激素接收细胞发出轴突？ 我们将联合收割机逆行 神经解剖技术与类固醇放射自显影在同一组织 为了观察孕激素敏感细胞是否将轴突发送到某些终端区域， 每一个都与脊柱前凸行为有关。 体外单单位电生理学是一种化学清洁的方法， 研究激素对下丘脑神经元的影响。 我们将寻找 通过给予不同的E或E+P， 体内剂量，脊柱前凸测试，下丘脑腹内侧研究 对行为活性肽和递质的单一单位反应。 激素的超微结构效应提供了指导生理和 行为实验 我们将用电子显微镜观察 雌激素和孕激素对下丘脑神经元的作用， 允许激素治疗之间的形态/行为相关性 持续时间或数量的受体位点占领。 下丘脑神经元 还将检查其他脑区域中的轴突终末。 我们已经开发了原位杂交技术的应用， 脑组织，用于使用标记的cDNA检测脑组织中的特异性信使RNA， 单独的细胞群。 我们将用这种技术来研究激素 对核糖体RNA和信使RNA水平的影响， 前脑啡肽