ANTIFERTILITY EFFECTS OF RESIDUAL AND EXCRETED GOSSYPOL

目前FDA关于肉类饮食中棉酚允许水平的指南 动物和人类是基于产生某些 毒性反应。 现在知道棉酚作为雄性是高度有效的 抗生育剂。 然而，通过摄入棉酚的微妙影响， 其在动物组织中的残留存在从未被研究过。 同样，没有关于潜在环境影响的信息 在食用棉酚的动物的粪便中排出的棉酚。尽管如此， 缺乏知识，棉籽粕（其中含有高水平的 棉酚）在全世界范围内广泛用于动物和人类的饮食中 (35%的棉籽粕被单胃动物消耗）。 我们 拟议的研究将确定摄入FDA的直接影响， 棉酚（450ppm）对雄性单胃动物生育力的允许水平 （公猪）消耗它和残留棉酚的影响， 组织和粪便对实验动物（雄性大鼠）生育力的影响。 我们 将制定这些提取程序的方法。 一个美国地方检察官。 实验室已同意免费提供必要的棉酚， 试点研究，以验证我们的技术，也已同意验证 我们提取程序的有效性 几种体内和体外研究 我们实验室以前曾开发过雄性大鼠的生育力测试。 我们的体外测试具有高度敏感性，能够检测出 从组织中提取的少量残留棉酚的影响， 快速产生结果。 这结合了个人的专业知识， 兽医科学、动物科学和肉类科学技术， 进行所有许多活动所需的技能、设施和设备 这项研究的各个方面。 这项研究将提供信息，以评估 含有棉酚残留的肉类的安全性， 排泄棉酚，提供新的信息，抗生育作用 并为监管机构提供新的信息。

Present FDA guidelines as to allowable level of gossypol in diets of meat animals and humans were established based upon levels which yielded certain toxic reactions. It is now known that gossypol is highly potent as a male antifertility agent. However, the subtle effects of gossypol ingestion via its residual presence in animal tissue has never been investigated. Likewise, there is no information as to the potential environmental impact of gossypol excreted in the feces of animals consuming it. Despite this paucity of knowledge, cottonseed meal (which contains high level of gossypol) is widely used throughout the world in animal and human diets (35% of cottonseed meal produced is consumed by monogastrics). Our proposed study will determine the direct effects of ingestion of the FDA allowable level of gossypol (450 ppm) on fertility of male monogastrics (boars) consuming it and the effects of residual gossypol extracted from tissue and feces on the fertility of laboratory animals (male rats). We will develop the methodology for these extraction procedures. A U.S.D.A. laboratory has agreed to provide free of charge the gossypol necessary for the pilot studies to validate our technique and also has agreed to verify the validity of our extraction procedures. Several In Vivo and In Vitro tests of fertility in male rats have been previously developed by our lab. Our In Vitro tests are highly sensitive and capable of detecting the effects of small amounts of residual gossypol extracted from tissue and yield a rapid result. This combines the expertise of individuals in veterinary science, animal science and meat science and technology who have the requisite skills, facilities and equipment to conduct all of the many aspects of this study. This study will provide information to assess the safety of meat containing gossypol residues, the biological activity of excreted gossypol, provide new information as to the antifertility effects of gossypol and supply new information for regulatory agencies.