NEURAL INFLUENCE ON HYPOPHYSEAL-GONADAL FUNCTION

促性腺激素释放激素(GnRH)可能由下丘脑分泌 神经元在不同的周期性脉冲中。然后GnRH的这些有节奏的爆发 启动促性腺激素的分泌模式。频率和频率 这些神经和垂体事件的幅度依次受以下因素影响 两种卵巢类固醇激素雌二醇和雌二醇组的动态变化 孕激素。仍然需要澄清的是，在 这个过程，其他体液因子的身份(催乳素， 可能改变这些事件的单胺类药物或阿片类药物)及其机制 行动的一部分。催乳素释放因子(PRF)的同源性及其作用方式 分泌物，以及神经化学事件和神经解剖学区域 调节催乳素的分泌也必须研究。具有特定的 促性腺激素、类固醇、催乳素和促性腺激素释放激素的放射免疫分析 输液-提取装置的类型，以及125I标记的检测方法 垂体细胞膜上的GnRH，我们建议确定GnRH的分泌 和GnRH受体结合特性；来关联这些变量 促性腺激素分泌；并确定雌激素和 黄体酮对猕猴的作用。来探索这样一种假设：神经 GnRH以外的多肽也是有节奏地分泌的，我们将研究 神经化学事件和神经解剖学区域控制 黄体生成素和催乳素的同步释放。神经 去传入、局部损毁、电刺激、垂体柄 将采用横切术、脑垂体术和脑垂体移植。 与激素和药物治疗相结合。最后，我们希望检查一下 通过激素和药物治疗来释放催乳素。最后，我们希望 在生理状态下检测催乳素和黄体生成素的释放 催乳素的释放和黄体生成素的释放可能不会发生。因此，我们将监控 婴幼儿哺乳过程中血清催乳素和黄体生成素的变化及比较 垂体柄切断后和垂体后的变化 移植。这些研究将提供第一个直接证据，证明 控制清醒灵长类动物GnRH分泌的事件，并将增强我们的 对控制促性腺激素的神经和激素事件的理解 和催乳素的分泌。这一知识可能有助于确定 高催乳素血症和促性腺激素减退及其潜在的相互作用 导致生育率下降。

Gondatropin-releasing hormone (GnRH) is probably secreted from hypothalamic neurons in distinct, periodic pulses. These rhythmic bursts of GnRH then initiate the secretion patterns of gonadotropins. The frequency and amplitude of these neural and pituitary events are influenced, in turn, by the changing ciruclating levels of two ovarian steroids, estradiol and progesterone. Still to be clarified are the specific cellular events in this process, the identity of the other humoral agents (prolactin, monoamines, or opiates) that may alter these events, and their mechanisms of action. The identity of prolactin-releasing factor (PRF), its mode of secretion, and the neurochemical eents and neuroanatomical regions that regulate prolactin secretion must also be studied. With specific radioimmunoassays for gonadotropins, steroids, prolactin, and GnRH, two types of infusion-withdrawal devices, and an assay to measure 125I-labeled GnRH on pituitary cell membranes, we propose to determine GnRH secretion and GnRH receptor binding characteristics; to correlate these variables with gonadotroping secretion; and to determine the sites of estrogen and progesterone action in macaques. To explore the hypothesis that neural peptides other than GnRH are also secreted rhythmically, we will study the neurochemical events and neuroanatomical regions that control the synchronous release of luteinizng hormone (LH) and prolactin. Neural deafferentation, local lesioning, electrical stimulation, pituitary stalk transection, hypophysectomy, and hypophyseal transplantation will be used in conjunction with hormone and drug therapy. Finally, we wish to examine the release of prolactin with hormone and drug therapy. Finally, we wish to examine the release of prolactin and LH in a physiologic state in which prolactin release occurs and LH release may not. Thus, we will monitor prolactin and LH changes in serum during infant suckling and compare them with the changes after hypophyseal stalk transection and after pituitary transplantation. The studies will provide the first direct evidence of the events that control GnRH secretion in awake primates and will enhance our understanding of the neural and hormonal events controlling gonadotropin and prolactin secretion. This knowledge may help identify the causes of hyperprolactinemia and hypogonadotropism and their potential interactions in reduced fertility.