FSH AND THE REGULATION OF OVARIAN FOLLICULOGENESIS

哺乳动物卵泡发生是一系列精确的发育过程， 这需要几种细胞类型的合作努力， 一组复杂的化学信息的整合， 卵母细胞的成功成熟和排卵。 在过去几 几十年来，人们已经清楚地认识到，代谢过程中的许多步骤 卵巢内的通路受卵巢外的联合控制， （垂体）和卵巢内因素。 本报告的总体目标 建议是研究荷尔蒙机制的基本方面， 负责卵泡生成。 这项建议涉及到 促卵泡激素（FSH）的作用机制及其在 早期卵泡形成 特别是FSH的细胞机制 引起的卵巢颗粒细胞功能的改变。 FSH结合、内化和随后的 在类固醇分泌和LH启动中的细胞内加工 将评价受体诱导。 在此概述的实验 建议将：1）分析内在化和细胞的结合 在活颗粒细胞中处理FSH的荧光衍生物 使用时间推移视频图像增强显微镜; 2）确定在 FSH受体的分布和转归 3）检测FSH受体的作用 LH/hCG受体的动态。

Mammalian ovarian folliculogenesis is a precise series of developmental events that requires the cooperative efforts of several cell types and the integration of a complex set of chemical messages that ultimately lead to successful maturation and ovulation of the oocyte. Within the past few decades it has become clear that many of the steps in the metabolic pathways within the ovary are under the combined control of extraovarian (hypophyseal) and introvarian factors. The overall objective of this proposal is to examine fundamental aspects of the hormonal mechanisms responsible for folliculogenesis. This proposal is concerned with the mechanism of action of follicle stimulating hormone (FSH) and its role in early folliculogenesis. Specifically the cellular mechanism underlying FSH induced alteration of ovarian granulosa cell function will be addressed. The functional role of FSH binding, internalization and subsequent intracellular processing in the initiation of steroid secretion and LH receptor induction will be evaluated. Experiments outlined in this proposal will: 1) analyze the binding of internalization and cellular processing of fluorescent derivatives of FSH in living granulosa cells using time lapse video image intensification microscopy; 2) determine at the ultrastructural level the distribution and fate of the FSH receptor following hormone binding; and 3) examine the effect of FSH receptor occupancy on the dynamics of the LH/hCG receptor.