FSH AND THE REGULATION OF OVARIAN FOLLICULOGENESIS

哺乳动物的卵泡发生是一系列精确的发育过程 需要几种细胞类型的合作努力的事件 整合一组复杂的化学信息，最终导致 卵母细胞成功成熟和排卵。在过去的几年里 几十年来，很明显，新陈代谢中的许多步骤 卵巢内的通路受卵巢外的共同控制 (垂体腺)和内分泌因子。这样做的总体目标是 建议是检查荷尔蒙机制的基本方面 负责卵泡的发生。这项提议涉及到 卵泡刺激素(FSH)的作用机制及其在糖尿病中的作用 早期卵泡发生。特别是卵泡刺激素的细胞机制 引起的卵巢颗粒细胞功能改变将被讨论。 促性腺激素结合、内化及随后的功能作用 类固醇分泌和促黄体生成素启动的细胞内处理 将对受体诱导进行评估。这篇文章中概述的实验 建议：1)分析内化与细胞的结合 卵泡刺激素荧光衍生物在活体颗粒细胞中的加工 使用延时视频图像增强显微镜；2)测定 卵泡刺激素受体的超微结构及其分布和去向 激素结合后；以及3)检测FSH受体的作用 黄体生成素/人绒毛膜促性腺激素受体的动态变化。

Mammalian ovarian folliculogenesis is a precise series of developmental events that requires the cooperative efforts of several cell types and the integration of a complex set of chemical messages that ultimately lead to successful maturation and ovulation of the oocyte. Within the past few decades it has become clear that many of the steps in the metabolic pathways within the ovary are under the combined control of extraovarian (hypophyseal) and introvarian factors. The overall objective of this proposal is to examine fundamental aspects of the hormonal mechanisms responsible for folliculogenesis. This proposal is concerned with the mechanism of action of follicle stimulating hormone (FSH) and its role in early folliculogenesis. Specifically the cellular mechanism underlying FSH induced alteration of ovarian granulosa cell function will be addressed. The functional role of FSH binding, internalization and subsequent intracellular processing in the initiation of steroid secretion and LH receptor induction will be evaluated. Experiments outlined in this proposal will: 1) analyze the binding of internalization and cellular processing of fluorescent derivatives of FSH in living granulosa cells using time lapse video image intensification microscopy; 2) determine at the ultrastructural level the distribution and fate of the FSH receptor following hormone binding; and 3) examine the effect of FSH receptor occupancy on the dynamics of the LH/hCG receptor.