REPRODUCTION--PINEAL GRAFTS, LH, FSH, AND PROLACTIN

生殖——松果体移植物、LH、FSH 和催乳素

• 批准号: 3325552
• 负责人: MARCIA G WELSH
• 金额: $ 9.98万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3325552
• 关键词: cell type; cerebrospinal fluid; electron microscopy; endocrine transplantation; estrus; follicle stimulating hormone; gerbil /jird; hamsters; hormone regulation /control mechanism; immunocytochemistry; innervation; luteinizing hormone; melatonin; pineal body; pituitary gonadal axis; prolactin; radiography; radioimmunoassay; reproduction; testis

The mammalian pineal complex has been widely studied as an integral component of the neuroendocrine-reproductive axis. Although he rodent pineal is divided into superficial and deep components, only the superficial gland has been studied in detail. The deep pineal of rodents is in immediate contact with the third ventricle and has morphological features that indicate interaction with the cerebrospinal fluid (CSF). This proposal addresses the question of how the pineal gland interacts with the CSF in both the intact system and in pineal grafts placed into the third ventricle. Pineal grafts will also be used to study the effects of the pineal on LH, FSH, and prolactin secretion from the intact adenohypophysis as well as from the adenohypophysis when it is co-transplanted with pineal glands beneath the renal capsule or into the hamster cheek pouch. The specific aims of this proposal are to test the following hypotheses: (1) There are structural relationships within the pineal complex which form an anatomical basis to explain potentia interaction between CSF-contacting pinealocytes, non-CSF- contacting parenchymal cells of the deep pineal and cells of the superficial pineal, (2) Pinealocytes have the ability to take up substances in the CSF but only if they display the morphology of normal CSF-contacting pinealocytes; CSF-contacting pinealocytes in situ can transport substances from the CSF to the superficial pineal, indicating the existing of a pathway for the potential transport of substances between non-CSF-contacting pineal parenchymal cells and the CSF; (3) Superficial pineal grafts in the third ventricle will display normal pinealocyte morphology and secret melatonin but only if reinnervated by catecholaminergic neurons; such grafts will not display a circadian rhythm in melatonin secretion but they will decrease LH, FSH and prolactin secretion, suppress estrous cyclicity and result in testicular regression; and (4) The superficial pineal gland transplanted to the periphery will secrete melatonin which will stimulate prolactin but not LH and FSH secretion but only if reinnervated by catecholaminergic neurons. Techniques to be used include light and electron microscopy techniques including autoradiography (3H- melatonin) and immunohistochemistry (S-ag, LH, FSH, prolactin, tyrosine hydroxylase), 3-dimensional computer reconstruction of pinealocytes, morphometric analyses, and radioimmunoassays (LH, FSH and prolactin). The present proposal will contribute valuable information regarding CSF-pineal as well as pineal-adenohypophyseal interactions and their effects on the reproductive system.

哺乳动物的松果体复合体作为一个整体被广泛研究 神经内分泌-生殖轴的组成部分。 虽然他 啮齿动物松果体分为表层和深层成分， 对浅表腺体进行了详细的研究。 深层松果体 的啮齿动物与第三脑室直接接触， 形态学特征表明与 脑脊液（CSF）。 这一建议解决了以下问题 松果体如何与脑脊液相互作用， 系统和松果体移植物放置到第三脑室。 松果体移植也将用于研究松果体的影响， 对完整腺垂体LH、FSH和催乳素分泌的影响 以及从腺垂体，当它是共同移植与 松果体位于肾包膜下或仓鼠脸颊内 邮袋。 本提案的具体目的是测试 以下假设：（1）存在结构关系 在松果体复合体中形成了一个解剖学基础， 与CSF接触的松果体细胞、非CSF- 接触深层松果体的实质细胞和 （2）松果体细胞具有摄取 CSF中的物质，但只有当它们显示出 正常接触脑脊液的松果体细胞 原位可以将物质从CSF运输到浅表 松果体，表明潜在的途径的存在 非接触脑脊液的松果体之间物质的运输 （3）松果体浅层移植物 第三脑室将显示正常的松果体细胞形态， 分泌褪黑激素，但只有当由儿茶酚胺能神经再支配时 神经元;这样的移植物将不会显示昼夜节律， 褪黑激素分泌，但它们会降低LH、FSH和催乳素 分泌，抑制发情周期，导致睾丸 退化;和（4）表浅松果体移植到 外周将分泌褪黑激素，褪黑激素将刺激催乳素 而不是LH和FSH的分泌，但只有当 儿茶酚胺能神经元 将使用的技术包括光和 电子显微镜技术，包括放射自显影（3 H- 褪黑激素）和免疫组织化学（S-ag，LH，FSH，催乳素， 酪氨酸羟化酶），三维计算机重建， 松果体细胞、形态测定和放射免疫测定（LH、FSH 和催乳素）。 目前的建议将有助于宝贵的 关于CSF-松果体以及松果体-腺垂体的信息 相互作用及其对生殖系统的影响。