FURTHER ANALYSIS OF HUMAN SPERM FERTILIZING ABILITY

去透明带仓鼠卵的体外受精最初是 是我们提出来测试人类精子可再生性的 我们有 随后提出的数据，以建立使用这样的精子， 渗透试验，以区分可育和潜在 不育或低生育力精液样本。 许多调查人员 验证了这种体外受精程序的能力， 评估精液质量。 这项测试似乎能够评估 人类精子进行获能，顶体反应， 和卵子融合 本提案的目的是研究 目前可用于不孕症的各种治疗的有效性 男性 对男性不育症治疗方式的评估将 涉及使用无透明带卵穿透试验作为 治疗前后的生育状况。 男性精索静脉曲张和 不孕症是要测试之前和之后手术结扎的 精索内静脉 克罗米芬治疗慢性阻塞性肺疾病的疗效观察 将通过双盲交叉研究进行评价， 不孕症患者 去除或减少白色血细胞， 低生育力样本将通过抗生素治疗和 物理去除细胞。 白色血细胞的功效 将通过监测常规治疗的变化来评估移除治疗 无透明带卵子测试系统中的精液参数和穿透率。 第四种治疗方式将涉及在培养基中洗涤精子 这可能会增强它们的可移植性， 妻子授精和无透明带仓鼠授精 鸡蛋 对低生育力男性的治疗效果进行评估将有助于 以澄清这种治疗的有效性。 使用无透明带卵子检测 我们将消除女性伴侣的混淆变量， 让我们的结果不那么模棱两可

The in vitro fertilization of zona-free hamster eggs was originally proposed by us as a test for human sperm fertilizing ability. We have subsequently presented data to establish the use of such a sperm penetration assay to differentiate between fertile and potentially infertile or subfertile semen samples. Numerous investigators have verified the ability of such an in vitro fertilization procedure to evaluate semen quality. This test appears to be capable of assessing the ability of human spermatozoa to undergo capacitation, acrosome reaction, and ovum fusion. The purpose of this proposal is to study the effectiveness of various treatments currently available for the infertile male. The assessment of treatment modalities for infertile males will involve using the zona-free egg penetration assay as an indicator of fertility status before and after treatment. Men with varicocele and infertility are to be tested before and after surgical ligation of the internal spermatic vein. The effectiveness of clomiphene citrate therapy in men will be evaluated by doing a double blind crossover study with subfertile patients. Removal or reduction of white blood cells in subfertile samples will be accomplished by both antibiotic treatment and physical removal of the cells. The effectiveness of white blood cell removal as a treatment will be evaluated by monitoring changes in routine semen parameters and penetration rates in the zona-free egg test system. The fourth treatment modality will involve washing spermatozoa in media which potentially enhances their fertilizing ability and doing intrauterine insemination in the wife as well as insemination of zona-free hamster eggs. An evaluation of treatment efficacy in subfertile males should help to clarify the validity of such treatments. Using the zona-free egg assay we will eliminate the confounding variable of the female partner and thus make our results less equivocal.