HUMAN PLACENTAL ATRIAL NATRIURETIC FACTOR RECEPTORS

人胎盘心房利尿钠因子受体

• 批准号: 3322147
• 负责人: INDIRA SEN
• 金额: $ 14.77万
• 依托单位: CLEVELAND CLINIC FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3322147
• 关键词: affinity chromatography; antibody; antibody formation; atrial natriuretic peptide; autoradiography; chemical binding; clinical chemistry; cyclic GMP; electron microscopy; enzyme mechanism; gel electrophoresis; gel filtration chromatography; guanylate cyclase; hormone binding protein; hormone receptor; hormone regulation /control mechanism; human pregnant subject; human tissue; laboratory mouse; laboratory rabbit; membrane proteins; monoclonal antibody; phosphorylation; placenta; pregnancy disorder; pregnancy toxemia /hypertension; protein biosynthesis; protein structure; radioimmunoassay

Atrial natriuretic factor (ANF), a recently discovered polypeptide hormone, is one of the most potent naturally occurring natriuretic and diuretic agents. By analogy to other hormones, its cellular effects are probably mediated by ANF-receptor interactions. The aims of this research are to characterize biochemically, physicochemically, and immunologically ANF receptor from human placenta. We will also characterize the ANF-induced phosphorylation of an endogenous substrate which we have recently observed in placental membranes and establish its relationship to the receptor. We will purify the receptor by affinity chromatography; establish optimal conditions and structural requirements for binding of the hormone to its receptors; determine its physicochemical structure; investigate the possibility of multiple binding site & prepare antibodies against receptor protein. Availability of purified receptor and antireceptor antibodies will enable us to fulfill our long-term goals of determining tissue, cellular, and subcellular distribution of atrial natriuretic factor receptors; elucidating its biosynthesis in human tissues; detecting structural differences in it associated with physiological or pathophysiological conditions such as pregnancies complicated with hypertension, and perhaps intervening therapeutically in the pathogenic process to which it contributes.

心房钠尿因子（ANF），一种最近发现的多肽 激素，是最有效的天然利钠剂之一 和利尿剂。 与其他激素类比，它的细胞 效应可能是由 ANF-受体相互作用介导的。 这 这项研究的目的是从生化角度表征， 物理化学和免疫学上的 ANF 受体 人类胎盘。 我们还将描述 ANF 引起的 我们拥有的内源底物的磷酸化 最近在胎盘膜中观察到并确定其 与受体的关系。 我们将通过以下方式纯化受体 亲和层析；建立最佳条件并 激素与其结合的结构要求 受体；确定其物理化学结构；调查 多个结合位点的可能性并制备抗体 对抗受体蛋白。 纯化受体的可用性和 抗受体抗体将使我们能够实现我们的长期目标 确定组织、细胞和亚细胞分布的目标 心房钠尿因子受体；阐明其生物合成 在人体组织中；检测其相关的结构差异 具有生理或病理生理状况，例如 怀孕并发高血压，也许 治疗性干预其致病过程 做出贡献。

项目成果

Angiotensin II-binding protein in adult and neonatal rat heart.

成年和新生大鼠心脏中的血管紧张素 II 结合蛋白。

• DOI: 10.1016/0022-2828(91)90048-q
• 发表时间: 1991
• 期刊: Journal of molecular and cellular cardiology
• 影响因子: 5
• 作者: Sen,I;Rajasekaran,AK
• 通讯作者: Rajasekaran,AK

Establishment of transfected cell lines producing testicular angiotensin-converting enzyme. Structural relationship between its secreted and cellular forms.

建立产生睾丸血管紧张素转换酶的转染细胞系。

• 发表时间: 1991
• 期刊: The Journal of biological chemistry
• 作者: Sen,I;Samanta,H;Livingston3rd,W;Sen,GC
• 通讯作者: Sen,GC