HUMAN PLACENTAL ATRIAL NATRIURETIC FACTOR RECEPTORS

人胎盘心房利尿钠因子受体

• 批准号: 3322146
• 负责人: INDIRA SEN
• 金额: $ 15.9万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3322146
• 关键词: affinity chromatography; antibody; antibody formation; atrial natriuretic peptide; autoradiography; chemical binding; clinical chemistry; cyclic GMP; electron microscopy; gel electrophoresis; gel filtration chromatography; guanylate cyclase; hormone regulation /control mechanism; human tissue; monoclonal antibody; phosphorylation; placenta; pregnancy disorder; pregnancy toxemia /hypertension; radioimmunoassay

Atrial natriuretic factor (ANF), a recently discovered polypeptide hormone, is one of the most potent naturally occurring natriuretic and diuretic agents. By analogy to other hormones, its cellular effects are probably mediated by ANF-receptor interactions. The aims of this research are to characterize biochemically, physicochemically, and immunologically ANF receptor from human placenta. We will also characterize the ANF-induced phosphorylation of an endogenous substrate which we have recently observed in placental membranes and establish its relationship to the receptor. We will purify the receptor by affinity chromatography; establish optimal conditions and structural requirements for binding of the hormone to its receptors; determine its physicochemical structure; investigate the possibility of multiple binding site & prepare antibodies against receptor protein. Availability of purified receptor and antireceptor antibodies will enable us to fulfill our long-term goals of determining tissue, cellular, and subcellular distribution of atrial natriuretic factor receptors; elucidating its biosynthesis in human tissues; detecting structural differences in it associated with physiological or pathophysiological conditions such as pregnancies complicated with hypertension, and perhaps intervening therapeutically in the pathogenic process to which it contributes.

心房利钠因子（ANF），最近发现的多肽 激素，是一种最有效的天然钠尿肽 和利尿剂。 与其他激素类似， 作用可能是由ANF-受体相互作用介导的。 的 本研究的目的是生物化学地表征， 从物理化学和免疫学角度来看， 人胎盘 我们还将描述ANF诱导的 内源性底物的磷酸化， 最近在胎盘膜中观察到， 与受体的关系。 我们将纯化受体， 亲和色谱法;建立最佳条件， 激素与其结合的结构要求 受体;测定其理化结构;研究 多结合位点可能性和制备抗体 针对受体蛋白。 纯化受体的可用性， 抗受体抗体将使我们能够实现我们的长期 确定组织、细胞和亚细胞分布的目标 心房利钠因子受体;阐明其生物合成 在人体组织中;检测其相关的结构差异 具有生理或病理生理条件，例如 妊娠合并高血压，也许 在治疗上干预其致病过程， 贡献。