EFFECTS OF PRENATAL SONOGRAPHY ON POSTNATAL DEVELOPMENT

本提案的长期目标是评估 妊娠期超声诊断 虽然好处和 诊断超声的优点被广泛地理解和接受， 一种产前诊断工具，目前尚不清楚诊断超声的使用 在怀孕期间对发育中的胎儿没有风险。 这种担忧是 由于最近引入了脉冲多普勒技术， 用于产前诊断 这种技术被用于第三 妊娠三个月，超声强度（SPTA）为5至10 比传统的产科CW多普勒大10倍。 由于其 由于结构和功能的复杂性，CNS被认为是 在产前后期容易受到有毒物质的伤害 发展 本提案的具体目的是评估 脉冲多普勒超声的强度与临床使用相当， 妊娠晚期胎鼠。 5个处理组，包括一个笼 对照组、假手术对照组和三种单独的超声强度， 用于研究设计。 分娩后， 将通过行为测试组合对治疗组进行评价，以评估： 1)妊娠结局，2）身体生长和成熟，3）反射和 运动发育，4）感觉功能，5）活动和反应水平， 6)学习和记忆能力，以及7）神经运动功能 系统. 这些研究将根据GLP指导原则进行，以确保 数据的质量和完整性在一个适合的框架下， 获得联邦监管机构（FDA）的认可。 结果从这些 研究将提供有关超声在治疗期间的生物效应的信息。 怀孕对产后发育的影响，并能够合理估计 超声波对发育中的生物体的生物效应和风险。

The longterm objectives of this proposal is to evaluate the safety of diagnostic ultrasound imaging in pregnancy. Although the benefits and advantages of diagnostic ultrasound are widely appreciated and accepted as a prenatal diagnostic tool it is not clear if diagnostic ultrasound usage during pregnancy is free of risk to the developing fetus. This concern is increased by the recent introduction of the pulse Doppler technique for prenatal diagnostic purposes. This technique is used in the third trimester of pregnancy and presents an ultrasound intensity (SPTA) 5 to 10 fold greater than traditional obstetrical CW Doppler. Because of its complexity of structure and function the CNS is recognized as especially susceptible to toxic insults during this later period of prenatal development. The specific aim of this proposal is to evaluate the effects of pulsed Doppler Ultrasound at intensities comparable to clinical use on third trimester fetal rats. Five treatment groups consisting of a cage control, a sham control and three separate ultrasound intensities will be used in the study design. Following parturition the pups from each treatment group will be evaluated by a behavioral test battery to assess: 1) pregnancy outcome, 2) physical growth and maturation, 3) reflex and motor development, 4) sensory function, 5) activity and reactivity levels, 6) learning and memory abilities, and 7) functioning in neuromotor systems. These studies will be conducted under GLP guidelines to assure the quality and integrity of the data under a framework suitable for acceptance to Federal Regulatory Agencies (FDA). The results from these studies will provide information on the bioeffects of ultrasound during pregnancy on postnatal development and enable reasonable estimates of bioeffects and risks of ultrasound on developing organism.