INFERTILITY--CELL-CELL REGULATION IN THE HUMAN OVARY

这一应用侧重于人类卵巢细胞-细胞调节机制 属于生化性质的，涉及到用人类颗粒进行实验 来自体外受精(IVF)程序的细胞。工作范围 将强调生长因子的生理作用和假定的 细胞自身在细胞上产生的自分泌/旁分泌因子 体外生长和分化。具体目标是：1)分离 并根据颗粒细胞的密度、形态、 有丝分裂活性、生殖激素(促性腺激素、雌二醇)受体 无血清和无血清中类固醇激素的分布或功能 含血清培养液，2)详细鉴定祖细胞 在无血清条件下有丝分裂活跃的亚型，以及3)至 调查某些生长因子是否是由 无血清条件下特定的颗粒细胞亚型。《长河》 本研究的学期目标是提供以下信息 依赖内分泌的细胞-细胞调节机制，如生长 人卵巢内的因子-促性腺激素相互作用 适用于排卵障碍等临床重大问题 和囊肿性疾病。这项工作应该提供新的概念，以便 了解这些疾病的基础。

This application focuses upon human ovarian cell-cell regulatory mechanisms of a biochemical nature and involves experimentation with human granulosa cells derived from in vitro fertilization (IVF) procedures. The work scope will emphasize the physiological effects of growth factors and of putative autocrine/paracrine factors produced by the cells themselves upon cellular growth and differentiation in vitro. Specific aims are: 1) to separate and characterize granulosa cell sub-types according to density, morphology, mitotic activity, reproductive hormone (gonadotropin, estradiol) receptor distribution or function, and steroidogenesis in serum-free and serum-containing medium, 2) to characterize in detail a progenitor cell sub-type which is mitotically active under serum-free conditions, and 3) to investigate whether certain growth factors are selectively produced by specific granulosa cell sub-types under serum-free conditions. The long term goal of this research is to provide information about endocrine-dependent cell-cell regulatory mechanisms such as growth factor-gonadotropin interactions within the human ovary which will be applicable to clinically significant problems such as ovulatory disorders and cystic conditions. This work should provide new concepts with which to understand the basis of these disorders.