INFERTILITY--CELL-CELL REGULATION IN THE HUMAN OVARY

不孕症——人类卵巢中的细胞调节

• 批准号: 3320057
• 负责人: DAVID W SCHOMBERG
• 金额: $ 15.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3320057
• 关键词: cell differentiation; cell growth regulation; cell type; deficient growth media; estradiol; female reproductive system disorder; fertility; growth factor; high performance liquid chromatography; histochemistry /cytochemistry; hormone regulation /control mechanism; human tissue; immunochemistry; in vitro fertilization; insulin; monoclonal antibody; ovarian cysts; ovary; ovulation; platelet derived growth factor; polycystic ovary syndrome; radiotracer; serum; steroid biosynthesis

This application focuses upon human ovarian cell-cell regulatory mechanisms of a biochemical nature and involves experimentation with human granulosa cells derived from in vitro fertilization (IVF) procedures. The work scope will emphasize the physiological effects of growth factors and of putative autocrine/paracrine factors produced by the cells themselves upon cellular growth and differentiation in vitro. Specific aims are: 1) to separate and characterize granulosa cell sub-types according to density, morphology, mitotic activity, reproductive hormone (gonadotropin, estradiol) receptor distribution or function, and steroidogenesis in serum-free and serum-containing medium, 2) to characterize in detail a progenitor cell sub-type which is mitotically active under serum-free conditions, and 3) to investigate whether certain growth factors are selectively produced by specific granulosa cell sub-types under serum-free conditions. The long term goal of this research is to provide information about endocrine-dependent cell-cell regulatory mechanisms such as growth factor-gonadotropin interactions within the human ovary which will be applicable to clinically significant problems such as ovulatory disorders and cystic conditions. This work should provide new concepts with which to understand the basis of these disorders.

本申请的重点是人类卵巢细胞-细胞调节机制 生物化学性质，涉及人体颗粒实验 来自体外受精（IVF）程序的细胞。 工作范围 将强调生长因子的生理作用和假定的 自分泌/旁分泌因子由细胞自身产生， 体外生长和分化。 具体目标是：1）分离 并根据颗粒细胞的密度，形态， 有丝分裂活性，生殖激素（促性腺激素，雌二醇）受体 分布或功能，以及无血清和 含血清培养基，2）详细表征祖细胞 在无血清条件下有丝分裂活性的亚型，和3） 研究某些生长因子是否是由 无血清条件下的特定颗粒细胞亚型。 长 这项研究的长期目标是提供有关 内分泌依赖的细胞-细胞调节机制，如生长 人类卵巢内的促性腺激素相互作用， 适用于临床上重要的问题，如排卵障碍 和囊性疾病 这项工作应提供新的概念， 了解这些疾病的基础。