GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO

体内和体外促性腺激素受体调节

• 批准号: 3311680
• 负责人: DAVID W SCHOMBERG
• 金额: $ 15.54万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311680
• 关键词: androgen inhibitor; androgens; chorionic gonadotropin; cyclic AMP; dihydrotestosterone; epidermal growth factor; estradiol; estrone; follicle stimulating hormone; genital secretion; graafian follicles; high density lipoproteins; histology; hormone inhibitor; hormone regulation /control mechanism; hypophysectomy; low density lipoprotein; luteinizing hormone; ovary; pregnancy; progesterone; pseudopregnancy; radiotracer; steroid hormone biosynthesis; testosterone; tissue /cell culture

The long-term objective of the project is to provide a basic understanding of ovarian cellular gonadotropin receptor regulation which will eventually be applicable to the problem of fertility regulation. This application is concerned with three general issues which continue the long-term project objective and summarize/encompass the specific aims of this proposal: a) the role of certain growth factors in key ovarian cellular differentiation processes such as FSH-dependent LH receptor induction, b) whether gonadotropins and/or sex steroids might, in turn, modulate receptor populations for growth factors, and c) the use of serum-free culture conditions to study receptor regulation in intact cells. The specific aims of the project consist of the following experimental objectives: 1) to test homogenous prepatations of platelete-derived growth factor (PDGF) upon FSH-stimulated LH receptor induction and upon subsequent LH receptor-dependent cell functions in porcine and rat granulosa cell monolayers, 2) to examine the possibility that a non-PDGF component of platelet extracts might facilitate FSH-stimulated LH receptor induction and/or selected subsequent LH receptor dependent cell functions, 3) to characterize granulosa cell PDGF receptor binding and study PDGF receptor modulation by reproductive hormones in granulosa cell monolayers, 4) to characterize granulosa cell epidermal growth factor (EGF) receptor binding and study EGF receptor modulation by reproductive hormones in vivo and in vitro, and 5) to extend our study of the serum-free culture milieu and the performance of cultured granulosa cells by a) investigating the effects of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) upon growth factor/gonadotropin-stimulated steroidogenesis, and b) characterizing the responses in culture of granulosa cell sub-populations preferentially enriched or lacking in a particular membrane receptor.

该项目的长期目标是提供基本的了解 卵巢细胞促性腺激素受体的调节，最终将 适用于生育调节问题。 这个应用程序是 关注持续长期项目的三个一般性问题 目标并总结/涵盖该提案的具体目标：a) 某些生长因子在关键卵巢细胞分化中的作用 过程，例如 FSH 依赖性 LH 受体诱导，b) 是否 促性腺激素和/或性类固醇可能反过来调节受体 生长因子群体，以及 c) 无血清培养的使用 研究完整细胞中受体调节的条件。 具体目标 该项目包括以下实验目标：1） 测试血小板衍生生长因子 (PDGF) 的均质制备 FSH 刺激的 LH 受体诱导以及随后的 LH 猪和大鼠颗粒细胞的受体依赖性细胞功能 单层细胞，2) 检查非 PDGF 成分的可能性 血小板提取物可能促进 FSH 刺激的 LH 受体诱导 和/或选定的后续 LH 受体依赖性细胞功能，3) 表征颗粒细胞 PDGF 受体结合并研究 PDGF 受体 颗粒细胞单层中生殖激素的调节，4) 表征颗粒细胞表皮生长因子 (EGF) 受体结合 并研究体内和体内生殖激素对 EGF 受体的调节作用 体外，5）扩展我们对无血清培养环境和 通过 a) 研究培养的颗粒细胞的性能 低密度脂蛋白（LDL）和高密度脂蛋白（HDL） 生长因子/促性腺激素刺激的类固醇生成，和 b) 表征颗粒细胞亚群培养物的反应 优先富集或缺乏特定的膜受体。