GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
批准号:
3311682
负责人:
DAVID W SCHOMBERG
金额:
$24.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1978
资助国家:
美国
项目状态:
已结题
起止时间:
1978-04-01 至 1991-11-30
关键词:
aminoacid analyzer chorionic gonadotropin cyclic AMP epidermal growth factor follicle stimulating hormone gel electrophoresis gonadotropins graafian follicles granulosa cell high performance liquid chromatography hormone binding protein hormone biosynthesis hormone receptor hormone regulation /control mechanism immunochemistry laboratory rabbit laboratory rat luteinizing hormone monoclonal antibody neoplastic cell culture for noncancer research ovary platelet derived growth factor protein sequence radiotracer swine tissue /cell culture transforming growth factors tritium
中文摘要
该项目的长期目标是提供基本的
了解卵巢细胞促性腺激素受体调节
这最终将适用于生育问题
调控 本申请主要涉及
确定化学和生物特性的一个
已从血小板衍生物中部分纯化的因子
生长因子(PDGF)制剂。 这种材料有助于
通过增强FSH刺激的LH受体分化
颗粒细胞中的诱导。 它似乎是一种独特的
人类转化生长因子β
暂时命名为转化生长因子β样
材料(TGF-B-LM)。 为了推广这些发现,本建议
涉及三个具体目标:1)阐明化学
通过a)将其纯化至电泳,
B)确定所述多肽的氨基酸序列,
材料的气相微测序仪技术,和c)通过
产生用于纯化的TGF-B-LM抗体
方法:2)研究TGF-β的生物学特性,
a)更详细地分析TGF-β-LM如何增强
FSH刺激的功能性LH/hCG受体的诱导,B)
表征其生长促进或抑制特性,
颗粒细胞或卵丘细胞和卵巢癌的原代培养物
c)利用TGF-β-LM和抗TGF-β-LM
免疫沉淀方法来确定卵巢是否
细胞本身产生TGF-β-LM; 3)分离和鉴定一种
来自猪的FSH和EGF调节的Mr 27,000组分
颗粒细胞,并决定其主要生理作用。
英文摘要
The long-term objective of the project is to provide a basic
understanding of ovarian cellular gonadotropin receptor regulation
which will eventually be applicable to the problem of fertility
regulation. This application is concerned primarily with
determining the chemical and biological characteristics of a
factor which has been partially purified from platelet-derived
growth factor (PDGF) preparations. This material facilitates
differentiation by enhancing FSH-stimulated LH receptor
induction in granulosa cells. It appears to be a unique species of
human Transforming Growth Factor Beta which we are
provisionally designating Transforming Growth Factor-Beta-Like
Material (TGF-B-LM). To extend these findings, this proposal is
concerned with three specific aims: 1) to elucidate the chemical
properties of TGF-B-LM by a) purifying it to electrophoretic
homogeneity, b) determining the amino acid sequence of the
material by gas-phase microsequenator technology, and c) by
generating antibodies to TGF-B-LM for application to purification
procedures; 2) to investigate the biological properties of TGF-B-
LM by a) analyzing in greater detail how TGF-B-LM potentiates
FSH-stimulated induction of functional LH/hCG receptor, b)
characterizing its growth-promoting or inhibiting properties upon
primary cultures of granulosa or cumulus cells and ovarian cancer
cell lines and c) utilizing TGF-B-LM and anti-TGF-B-LM
immunoprecipitation approaches to determine whether ovarian
cells themselves produce TGF-B-LM; 3) to isolate and identify an
FSH- and EGF-modulated Mr 27,000 component from porcine
granulosa cells and determine its principal physiological role.
期刊论文(0)
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科研奖励(0)
会议论文
INSULIN AND IGF-1 SIGNALING IN OVARIAN CELLS
-
批准号:6536268
-
项目类别:
-
资助金额:$7.7万
-
财政年份:2001
-
负责人:DAVID W SCHOMBERG
-
依托单位:
INSULIN AND IGF-1 SIGNALING IN OVARIAN CELLS
-
批准号:6233079
-
项目类别:
-
资助金额:$7.7万
-
财政年份:2001
-
负责人:DAVID W SCHOMBERG
-
依托单位:
FOLLICULOGENESIS: IMMORTALIZED GRANULOSA CELL LINES
-
批准号:2761838
-
项目类别:
-
资助金额:$7.7万
-
财政年份:1999
-
负责人:DAVID W SCHOMBERG
-
依托单位:
FOLLICULOGENESIS: IMMORTALIZED GRANULOSA CELL LINES
-
批准号:6138808
-
项目类别:
-
资助金额:$7.7万
-
财政年份:1999
-
负责人:DAVID W SCHOMBERG
-
依托单位:
SYMPOSIUM AND STATE OF THE ART LECTURES
-
批准号:3435374
-
项目类别:
-
资助金额:$0.89万
-
财政年份:1990
-
负责人:DAVID W SCHOMBERG
-
依托单位:
INFERTILITY: CELL-CELL REGULATION IN THE HUMAN OVARY
-
批准号:3320058
-
项目类别:
-
资助金额:$14.76万
-
财政年份:1985
-
负责人:DAVID W SCHOMBERG
-
依托单位:
INFERTILITY--CELL-CELL REGULATION IN THE HUMAN OVARY
-
批准号:3320059
-
项目类别:
-
资助金额:$15.72万
-
财政年份:1985
-
负责人:DAVID W SCHOMBERG
-
依托单位:
INFERTILITY--CELL-CELL REGULATION IN THE HUMAN OVARY
-
批准号:3320057
-
项目类别:
-
资助金额:$15.55万
-
财政年份:1985
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:2196843
-
项目类别:
-
资助金额:$15.63万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:2196845
-
项目类别:
-
资助金额:$17.0万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:3311681
-
项目类别:
-
资助金额:$19.03万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:3311680
-
项目类别:
-
资助金额:$15.54万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:2196844
-
项目类别:
-
资助金额:$16.24万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:3311678
-
项目类别:
-
资助金额:$8.3万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:3311679
-
项目类别:
-
资助金额:$17.05万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO
-
批准号:3311674
-
项目类别:
-
资助金额:$23.04万
-
财政年份:1978
-
负责人:DAVID W SCHOMBERG
-
依托单位:
海外基金