RENAL HUMORAL FACTORS IN RENAL FUNCTION AND HYPERTENSION

这项建议考察了前列腺素(PG)和 全肾和离体肾的肾素(R)血管紧张素系统 肾小球。研究将使用以下正常动物进行 肾灌注压(RPP)在以下的急性或慢性变化 肾血流自动调节的范围。这两人可能扮演的角色 系统在调节肾脏血流量和钠排泄方面发挥作用 探索过了。 这些目标是通过三条相互关联的调查路线进行的。 第一行包括两个协议系列，用于确定 大鼠离体肾小球PG和R释放的特点 刺激后花生四烯酸(AA)的内源性释放 磷脂酶(系列1)或当PG和R的产生被 外源AA(系列2)。在二线调查中有三个系列 描述了一系列协议。第一个系列探讨肾血流。 RPP和RPP急剧下降后在完整动物体内的分布 PG和R在调节或调节分布中的作用。第二 序列与序列一相似，但检查肾血流分布。 在长期(短期和长期)RPP下降之后。在第三季中 在慢性减少后，将从狗身上分离出肾小球协议 在RPP或从假动物身上。这种隔离的制剂使我们能够 从表面和表面两方面探讨AA-PG-R级联中可能存在的缺陷 延髓旁肾小球。 第三条调查线由一系列协议组成 旨在确定单边削减RPP(两者)的影响 急性和慢性)由AII的产生所介导的血管张力变化 在同侧肾和对侧肾均可见PG。

This proposal examines the relationship between the prostaglandin (PG) and the renin (R) angiotensin system in the whole kidney and in isolated glomeruli. Studies will be carried out using normal animals following acute or chronic changes in renal perfusion pressure (RPP) within and below the range of renal blood flow autoregulation. The possible role these two systems play in modulating renal blood flow and sodium excretion will be explored. These objectives are pursued in three interrelated lines of investigation. The first line includes two series of protocols to determine the characteristics of PG and R release in isolated glomeruli elicited by endogenous release of arachadonic acid (AA) after stimulation of phospholipase (series 1) or when PG and R production are evoked by exogenous AA (series 2). In the second line of investigation three series of protocols are described. The first series explores renal blood flow distribution in the intact animal following acute reductions in RPP and the role of PG and R in mediating or modulating the distribution. The second series is similar to series one, but examines renal blood flow distribution after chronic (short and long term) reductions in RPP. In the third series of protocols glomeruli will be isolated from dogs after chronic decreases in RPP or from sham animals. This isolated preparation allows us to explore possible defects in the AA-PG-R cascade in both superficial and juxtamedullary glomeruli. The third line of investigation is composed of a series of protocols designed to determine the effects of unilateral reductions in RPP (both acute and chronic) changes in vascular tone mediated by generation of AII or PG in both the ipsilateral and contralateral kidneys.