ROLE OF PLASMA LIPOPROTEINS IN ATHEROSCLEROSIS

本研究的最终目的是要澄清这些关系 血浆脂蛋白和动脉粥样硬化之间的关系。 我们将通过以下方式实现这一目标： 研究各种物理化学和酶的因素影响， 血浆脂蛋白及其脂质在动脉中的滞留和蓄积 利用模型系统。 由于抗动脉粥样硬化作用对高 密度脂蛋白（HDL）可能与卵磷脂-胆固醇 酰基转移酶反应，我们打算提供进一步的基本信息 人血浆卵磷脂-胆固醇酰基转移酶的性质，特别是其 物理化学和免疫化学性质。 我们将研究 酶-脂蛋白相互作用中的各种载脂蛋白。 我们还打算 建立猪、鼠血清中酶的纯化方法， 为了便于研究酶促反应与 实验动物的HDL代谢。 此外，我们会继续研究 血浆脂蛋白与主动脉结缔组织的相互作用 在一些实施方案中，所述组合物包含糖胺聚糖、蛋白聚糖、弹性蛋白和胶原蛋白。 在这些相互作用中，表面发生的变化和 脂蛋白在循环过程中和循环后的内部性质 将系统地研究进入主动脉。

The ultimate goal of the proposed research is to clarify the relationships between plasma lipoproteins and atherosclerosis. We will approach this goal by investigating various physicochemical and enzymatic factors influencing the retention and accumulation of plasma lipoproteins and their lipids in arterial tissues by utilizing model systems. Since antiatherogenic effects on high density lipoproteins (HDL) may well be associated with the lecithin-cholesterol acyltransferase reaction, we intend to provide further information on the basic properties of human plasma lecithin-cholesterol acyltransferase, especially its physicochemical and immunochemical properties. We will study the roles of various apo-lipoproteins in the enzyme-lipoprotein interactions. We also intend to establish the method for enzyme purification from rat and swine sera in order to facilitate the study of the relationship between the enzymatic reaction and HDL metabolism in experimental animals. In addition, we will continue the study of the interactions of plasma lipoproteins with aortic connective tissue components, such as glycosaminoglycans, proteoglycans, elastin, and collagen. The effects, on these interactions, of the changes occurring in the surface and interior properties of the lipoproteins during their circulation and after their entry into the aorta will be systematically investigated.