RENAL HUMORAL FACTORS IN RENAL FUNCTION AND HYPERTENSION

本研究旨在探讨前列腺素（PG）与 在整个肾脏和离体肾脏中的肾素（R）血管紧张素系统 肾小球 将使用正常动物进行研究， 肾灌注压（RPP）的急性或慢性变化 肾血流自动调节范围。 这两个可能的角色 系统在调节肾血流和钠排泄中的作用将是 探讨了 这些目标是在三个相互关联的调查路线中实现的。 第一行包括两个系列的协议，以确定 在离体肾小球中的PG和R释放特性 内源性释放花生四烯酸（AA）刺激后， 磷脂酶（系列1）或当PG和R的生产引起的 外源性AA（系列2）。 在调查的第二个系列中， 的协议进行了描述。 第一个系列探讨肾血流 RPP急性降低后在完整动物中的分布， PG和R在介导或调节分布中的作用。 第二 系列与系列1相似，但检查肾血流分布 在RPP慢性（短期和长期）降低后。 第三辑 在慢性减少后，将从犬中分离肾小球 在RPP中或来自假手术动物。 这种分离的制备使我们能够 探索AA-PG-R级联反应中可能存在的缺陷， 延髓肾小球。 第三条调查线由一系列协议组成 旨在确定单方面削减《香港规划署建议方案》的影响（ 急性和慢性）由AII生成介导的血管张力变化 或同侧和对侧肾脏中的PG。

This proposal examines the relationship between the prostaglandin (PG) and the renin (R) angiotensin system in the whole kidney and in isolated glomeruli. Studies will be carried out using normal animals following acute or chronic changes in renal perfusion pressure (RPP) within and below the range of renal blood flow autoregulation. The possible role these two systems play in modulating renal blood flow and sodium excretion will be explored. These objectives are pursued in three interrelated lines of investigation. The first line includes two series of protocols to determine the characteristics of PG and R release in isolated glomeruli elicited by endogenous release of arachadonic acid (AA) after stimulation of phospholipase (series 1) or when PG and R production are evoked by exogenous AA (series 2). In the second line of investigation three series of protocols are described. The first series explores renal blood flow distribution in the intact animal following acute reductions in RPP and the role of PG and R in mediating or modulating the distribution. The second series is similar to series one, but examines renal blood flow distribution after chronic (short and long term) reductions in RPP. In the third series of protocols glomeruli will be isolated from dogs after chronic decreases in RPP or from sham animals. This isolated preparation allows us to explore possible defects in the AA-PG-R cascade in both superficial and juxtamedullary glomeruli. The third line of investigation is composed of a series of protocols designed to determine the effects of unilateral reductions in RPP (both acute and chronic) changes in vascular tone mediated by generation of AII or PG in both the ipsilateral and contralateral kidneys.