URINARY HCG EXPRESSION IN PREGNANCY LOSS

妊娠失败时尿液 HCG 的表达

• 批准号: 3330201
• 负责人: JOHN F O'CONNOR
• 金额: $ 9.68万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3330201
• 关键词: biomarker; blood chemistry; chorionic gonadotropin; diagnosis design /evaluation; diagnosis quality /standard; early diagnosis; elastases; environmental toxicology; enzyme linked immunosorbent assay; epidemiology; human pregnant subject; human subject; hybridomas; immunization; laboratory mouse; molecular cloning; monoclonal antibody; occupational hazard; peptide structure; pregnancy loss; pregnancy tests; radioimmunoassay; urinalysis

Early pregnancy loss is an important concern and the subject of current and planned studies of reproductive hazards in the work place. Its determination requires the measurement of urinary human chorionic gonadotropin (hCG). We have very recently discovered that many of the measurements of hCG may be highly inaccurate because of the presence of peptide bond nicks in the urinary hCG which makes the hormone invisible to the antibodies currently used for its measurement. Furthermore, preliminary gel blotting studies of both serum and urine of pregnant women indicate significant concentrations of nicked hCG may be present in the circulation. These findings require immediate verification to avoid the necessity of re-exploring the current and proposed studies with new measuring systems. The objectives of this proposal are (1) to develop standards for the nicked forms of hCG in order to evaluate current antibody libraries to select potential measurement systems; (2) to measure the expression of all forms of hCG present in existent early pregnancy loss urine specimens now being cognizant of the possible presence of substantial concentrations of nicked hormone; (3) to collect parallel serum and urine specimens throughout pregnancy and compare the concentrations of the forms of hCG present in both serum and urine (4) to produce monoclonal antibodies to nicked forms of hCG to improve its measurement by development of a specific IRMA to a unique epitope expressed by nicked forms of hCG.

早孕丢失是一个重要的问题，也是当前和 计划对工作场所的生殖危害进行研究。 其 测定需要测量尿人绒毛膜 促性腺激素（hCG）。 我们最近发现， hCG的测量可能非常不准确，因为存在 尿hCG中的肽键缺口，使激素不可见， 目前用于其测量的抗体。 此外，委员会认为， 孕妇血清和尿液的初步凝胶印迹研究 表明有缺口的hCG的显著浓度可能存在于 流通 这些调查结果需要立即核实，以避免 有必要重新探讨目前和拟议的研究与新的 测量系统。 这项建议的目的是：（1）为有缺口的建筑物制订标准， hCG的形式，以评估目前的抗体库，以选择 潜在的测量系统;（2）测量所有形式的表达 hCG存在于现有的早期妊娠丢失尿标本中， 认识到可能存在大量集中的刻痕 激素;（3）全程平行采集血清和尿液标本 怀孕和比较的浓度形式的hCG存在于 血清和尿液（4）产生针对切口形式的单克隆抗体 hCG，以改善其测量通过发展一个特定的IRMA， 由hCG的切口形式表达的独特表位。