URINARY HCG EXPRESSION IN PREGNANCY LOSS

妊娠失败时尿液 HCG 的表达

• 批准号: 2201216
• 负责人: JOHN F O'CONNOR
• 金额: $ 10.04万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2201216
• 关键词: biomarker; blood chemistry; chorionic gonadotropin; diagnosis design /evaluation; diagnosis quality /standard; early diagnosis; elastases; environmental toxicology; enzyme linked immunosorbent assay; epidemiology; human pregnant subject; human subject; hybridomas; immunization; laboratory mouse; molecular cloning; monoclonal antibody; occupational hazard; peptide structure; pregnancy loss; pregnancy tests; radioimmunoassay; urinalysis

Early pregnancy loss is an important concern and the subject of current and planned studies of reproductive hazards in the work place. Its determination requires the measurement of urinary human chorionic gonadotropin (hCG). We have very recently discovered that many of the measurements of hCG may be highly inaccurate because of the presence of peptide bond nicks in the urinary hCG which makes the hormone invisible to the antibodies currently used for its measurement. Furthermore, preliminary gel blotting studies of both serum and urine of pregnant women indicate significant concentrations of nicked hCG may be present in the circulation. These findings require immediate verification to avoid the necessity of re-exploring the current and proposed studies with new measuring systems. The objectives of this proposal are (1) to develop standards for the nicked forms of hCG in order to evaluate current antibody libraries to select potential measurement systems; (2) to measure the expression of all forms of hCG present in existent early pregnancy loss urine specimens now being cognizant of the possible presence of substantial concentrations of nicked hormone; (3) to collect parallel serum and urine specimens throughout pregnancy and compare the concentrations of the forms of hCG present in both serum and urine (4) to produce monoclonal antibodies to nicked forms of hCG to improve its measurement by development of a specific IRMA to a unique epitope expressed by nicked forms of hCG.

早期妊娠丢失是一个重要的问题，也是当前和 对工作场所生殖危害的计划研究。它的 检测需要测量尿液中的人绒毛膜 促性腺激素(HCG)。我们最近发现，许多 由于hCG的存在，hCG的测量可能非常不准确 尿中人绒毛膜促性腺激素的多肽键被划破，从而使激素看不见 目前用于其测量的抗体。此外， 孕妇血清和尿液凝胶印迹的初步研究 表明人绒毛膜促性腺激素可能存在显著浓度 发行量。这些发现需要立即核实，以避免 以新的视角重新探讨当前和提出的研究的必要性 测量系统。 这项提案的目标是：(1)为黑人族制定标准 为了评价目前人绒毛膜促性腺激素抗体文库的选择 潜在的测量系统；(2)测量所有形式的表达 人绒毛膜促性腺激素在现有的早期妊娠丢失尿样中的存在 认识到可能存在大量浓度的镍 激素；(3)在整个过程中收集平行的血清和尿样 比较人绒毛膜促性腺激素(HCG)在妊娠过程中的浓度 血清和尿液(4)产生抗镍单抗 通过将特定的IRMA发展为 人绒毛膜促性腺激素的刻痕形式表达的独特表位。