REDUCED LORDOSIS BEHAVIOR AFTER INTRACEREBRAL 8-OH DPAT

拟议的研究旨在确定CNS网站(S)的责任 对雌性大鼠性行为的抑制作用 5-HT1A激动剂，8-羟基-2-9(二正丙氨基)四氢萘(8-OH-DPAT)。 8-OH-DPAT抑制胞体/树突状5-HT1A自身受体的激活 5-羟色胺神经元的放电和区域内5-羟色胺的释放减少 被这些神经元所支配。在终末领域，5-HT1a受体 存在于突触后部位。尽管它也可能驻留在突触前 终末，5-HT1a受体不起终末作用 自身感受器。拟议研究的具体目标是：(1) 找出足以抑制女性性欲的神经区域 8-OH-DPAT治疗后的行为和(2)比较 该5-HT1a激动剂与其他5-HT1a激动剂和 5-HT1B-首选激动剂。推测的5-HT1A拮抗剂的作用 也将进行研究。将使用完整的、定期骑自行车的雌性老鼠 并将特别强调区分突触后 8-OH-DPAT的作用随后将被注入大脑内侧 基础下丘脑和内侧视前区，控制 雌性生殖进入中缝背核，其中含有5-羟色胺 细胞体。输液前后将对性行为进行监测。 这些研究将为今后的调查奠定基础 在确定5-HT1a的分子性质和功能后果时- 对性行为的中介控制。

The proposed research is designed to identify the CNS site(s) responsible for the inhibition of female rat sexual behavior after treatment with the 5-HT1A agonist, 8-hydroxy-2-9(di-n-propylamino) tetralin (8-OH-DPAT). Activation of somal/dendritic 5-HT1A autoreceptors by 8-OH-DPAT decreases the firing of 5-HT neurons and reduces the release of 5-HT in regions innervated by these neurons. In terminal fields, the 5-HT1A receptor exists at postsynaptic sites. Although it may also reside on presynaptic terminals, the 5-HT1A receptor does not function as the terminal autoreceptor. The specific aims of the proposed studies are: (1) to identify the neural areas sufficient for the inhibition of female sexual behavior following treatment with 8-OH-DPAT and (2) to compare the effectiveness of this 5-HT1A agonist with that of other 5-HT1A agonists and 5-HT1B-preferring agonists. The effects of putative 5-HT1A antagonists will also be studied. Intact, regularly cycling female rats will be used and special emphasis will be placed on differentiating the postsynaptic effects of 8-OH-DPAT will then be infused intracerebrally into the medial basal hypothalamus and medial preoptic area, terminal fields that control female reproduction, and into the dorsal raphe nucleus, which contains 5-HT cell bodies. Sexual behavior will be monitored before and after infusion. These studies will provide the foundation for future investigations aimed at identifying the molecular nature and functional consequences of 5-HT1A- mediated control of sexual behavior.