BIOTIN REQUIREMENTS OF INFANTS

婴儿的生物素需求

• 批准号: 3328622
• 负责人: Donald M Mock
• 金额: $ 12.96万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-15 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3328622
• 关键词: aminoacid metabolism; baby food; biotin; developmental nutrition; dietary supplements; fatty acid metabolism; feces analysis; gas chromatography mass spectrometry; human milk; infant human (0-1 year); longitudinal human study; nutrient requirement; nutrition related tag; propionyl coA carboxylase; thin layer chromatography; urinalysis; vitamin analog; vitamin deficiency; vitamin metabolism

DESCRIPTION (Principal Investigator's Abstract): The long-term goal of this project is to determine the biotin requirement of normal infants. The specific aims are 1) to accurately measure the total content of biotin and biotin analogs in infant formula, urine, and feces, 2) to determine biotin balance in normal, formula-fed infants fed formula with biotin supplementation at levels similar to human milk and commercial infant formula. Studies in this project are important because several lines of evidence suggest that biotin nutritional status may be inadequate in some normal infants and that the inadequacy could lead to important clinical sequelae that would not be recognized as biotin deficiency. A more sensitive, chemically specific biotin assay based on 125I-avidin and HPLC has been developed that would allow measurement of plasma biotin and urinary excretion of biotin and biotin analogs as well as a more accurate estimate of free techniques, biotin balance will be determined before 30 and after 60 days of age in normal infants fed an infant formula. Using these same assays, biotin nutritional status will be determined longitudinally from 7 to 112 days of age in infants fed formula supplemented to reach three different total biotin concentrations: 1) a concentration equal to the first quartile of the distribution of biotin concentrations in human milk, 2) a second concentration equal to the third quartile of the same distribution, and 3) a third concentration typical of commercial infant formula. In these longitudinal studies, biotin depletion at the tissue level will also be assessed in two ways: 1) Deficient activity of the biotin-dependent enzyme 3-methylcrotonyl-CoA carboxylase impairs metabolism of leucine and leads to abnormal excretion of 3-hydroxyisovaleric acid in the urine. Gas chromatography/mass spectrometry with deuterated 30HIV as internal standard will be used to quantitate 3-HIV excretion. 2) Deficient activity of the biotin-dependent enzyme propionyl-CoA carboxylase causes an increase in the percentage composition of odd chain saturated fatty acid in the phospholipid fraction of plasma. Fatty acid composition in plasma phospholipid will be measured by thin layer chromatography/gas chromatography.

描述（主要研究者的摘要）： 该项目是为了确定正常婴儿的生物素需求。 这 具体目的是1）准确测量生物素的总含量和 婴儿配方，尿液和粪便中的生物素类似物，2）确定生物素 在正常的，配方奶粉的婴儿中喂养生物素的配方奶粉 与人牛奶和商业婴儿类似的水平补充 公式。 该项目的研究很重要，因为几行 有证据表明，生物素营养状况可能不足 正常婴儿，不足可能导致重要的临床 后遗症不会被认为是生物素缺乏症。 更多 基于125i-Avidin和HPLC的敏感，化学特异性的生物素测定 已经开发了可以测量血浆生物素和 生物素和生物素类似物的尿液排泄以及更准确的 自由技术的估计，将在30之前确定生物素平衡 正常婴儿60天后喂养婴儿配方奶粉。 使用 这些相同的测定法将确定生物素营养状况 喂养公式的婴儿的7至112天纵向 补充以达到三种不同的总生物素浓度：1）a 浓度等于生物素分布的第一个四分位数 人乳的浓度，2）第二个浓度等于第三 相同分布的四分位数，3）典型的第三个浓度 商业婴儿配方。 在这些纵向研究中，生物素消耗 在组织水平上也将通过两种方式进行评估：1） 生物素依赖性酶的活性3-甲基氯菊酰-COA羧化酶 损害亮氨酸的代谢，并导致异常排泄 尿液中的3-羟基异甲酸。 气相色谱/质量 具有氘化30HIV作为内标的光谱法将用于 定量3-HIV排泄。 2）依赖生物素的活性不足 酶丙二酰辅酶A羧化酶导致百分比增加 奇数链饱和脂肪酸的组成在磷脂馏分中 血浆。 将测量血浆磷脂中的脂肪酸组成 通过薄层色谱/气相色谱法。