CHROMOSOME 21: LINKAGE MAP WITH INDEX MARKERS

21 号染色体：带有索引标记的连锁图谱

• 批准号: 3333653
• 负责人: STYLIANOS E ANTONARAKIS
• 金额: $ 15.62万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3333653
• 关键词: DNA; computer assisted sequence analysis; dinucleotide; gene duplication; genetic markers; genetic polymorphism; genome; genotype; human genetic material tag; human tissue; linkage mapping; nucleic acid sequence; polymerase chain reaction

Human chromosome 21 is the smallest human chromosome which comprises about 1.4% od human genome. In the last five years, many DNA polymorphisms have been mapped to this chromosome and two genetic linkage maps have been developed using large reference pedigrees. The average distance between DNA markers in the existing linkage maps is 6- 10 Cm. However, not all mapped DNA markers are adequately informative, easy to score and available, and few can be typed using the polymerase chain reaction. The goal of this proposal is to create a detailed linkage map of human chromosome 21 using index DNA markers that are highly informative (Het greater than 0.60) can be preferably typed with PCR and their density on chromosome 21 is such that the average distance between adjacent markers is less than 5 Cm.

人类21号染色体是人类最小的染色体，它包括 约占人类基因组的1.4%。在过去的五年里，许多DNA 该染色体和两个遗传基因已被定位为多态 利用大的参考家系开发了连锁图谱。这个 现有连锁图谱中DNA标记间的平均距离为6- 10厘米。然而，并不是所有绘制的DNA标记都提供了足够的信息， 容易得分和可用的，很少有可以用聚合酶分型的 连锁反应。这项提案的目标是创建一个详细的 使用索引DNA标记的人类21号染色体连锁图谱 信息量大(Het大于0.60)最好可以用 PCR及其在21号染色体上的密度是这样的：平均距离 相邻标记之间的距离小于5厘米。