CHROMOSOME 21: LINKAGE MAP WITH INDEX MARKERS

人类染色体21是最小的人类染色体，其包括： 约占人类基因组的1.4%。在过去的五年里，许多DNA 多态性已被映射到该染色体和两个遗传 利用大的参考谱系已经开发了连锁图谱。的 现有连锁图谱中DNA标记间的平均距离为6- 10个， 10厘米然而，并不是所有映射的DNA标记都具有足够的信息量， 容易得分和可用，很少可以使用聚合酶分型 链反应该提案的目标是建立一个详细的 人类21号染色体的连锁图，使用索引DNA标记， 高信息性（Het大于0.60）的人可以优选地用 PCR和它们在21号染色体上的密度是这样的， 相邻标记之间的距离小于5 Cm。

Human chromosome 21 is the smallest human chromosome which comprises about 1.4% od human genome. In the last five years, many DNA polymorphisms have been mapped to this chromosome and two genetic linkage maps have been developed using large reference pedigrees. The average distance between DNA markers in the existing linkage maps is 6- 10 Cm. However, not all mapped DNA markers are adequately informative, easy to score and available, and few can be typed using the polymerase chain reaction. The goal of this proposal is to create a detailed linkage map of human chromosome 21 using index DNA markers that are highly informative (Het greater than 0.60) can be preferably typed with PCR and their density on chromosome 21 is such that the average distance between adjacent markers is less than 5 Cm.