MAPPING AND CHARACTERIZING QUANTITATIVE TRAITS IN MAIZE

玉米数量性状的绘图和表征

• 批准号: 3333545
• 负责人: BENJAMIN BURR
• 金额: $ 21.84万
• 依托单位: ASSOCIATED UNIV-BROOKHAVEN NATL LAB
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-28 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3333545
• 关键词: Fungi; alleles; biological polymorphism; chemical structure function; corn; gene expression; gene mutation; genetic mapping; molecular cloning; molecular genetics; molecular pathology; nucleic acid sequence; plant diseases; plant genetics; plant proteins; plant virus; transposon /insertion element

Genes having small quantitative effects rather than major qualitative effects will be mapped and characterized. The hypothesis upon which this work is based suggests that "wild-type" genes are polymorphic and have varying but small effects on the phenotype of the individual carrying them. When they are mutant, these genes have major effects and show Mendelian inheritance. Experimental evidence supporting this idea is discussed. Maize is easily manipulated genetically and is a very polymorphic species and therefore lends itself to the characterization of quantitative trait loci. Two large recombinant inbred families have been used to construct a map that spans the entire genome. These families will be used first to map genetic factors affecting quantitative traits: recombination, disease resistance, plant characters, and response to the environment. Mapping should lead to the identification of genes which, when mutant, show Mendelian segregation. These genes will be molecularly cloned so that the correlation established by mapping can be proved by showing that the segregating "wild-type" alleles indeed have quantitative differences in their expression. While the characters studied here are of more interest to plant science and agronomy, the work has important implications for the study of quantitative factors of medical importance. Recombinant inbred lines have been established in mice, but maps associated with them are still incomplete. Mapping in human families likewise makes use of some of the same ideas that were established for recombinant inbred families. Maize is thus a model system where quantitative trait loci can more easily be mapped and characterized.

数量影响较小而非主要的基因 定性影响将被绘制和表征。 这 这项工作所依据的假设表明“野生型” 基因是多态性的，对基因有不同但很小的影响 携带它们的个体的表型。 当他们突变时， 这些基因具有重大影响并显示孟德尔遗传。 讨论了支持这一想法的实验证据。 玉米是 容易进行基因操纵，是一个多态性很强的物种 因此适合定量表征 性状基因座。 已使用两个大型重组近交系 构建涵盖整个基因组的图谱。 这些家庭 将首先用于绘制影响数量的遗传因素 性状：重组、抗病性、植物性状，以及 对环境的反应。 映射应该导致 鉴定突变时表现出孟德尔规律的基因 隔离。 这些基因将被分子克隆，以便 通过映射建立的相关性可以通过证明 分离的“野生型”等位基因确实具有定量 他们的表达方式的差异。 当人物在这里学习时 对植物科学和农学更感兴趣，这项工作 对定量因素研究具有重要意义 医学重要性。 重组自交系已建立 在小鼠中，但与它们相关的图谱仍然不完整。 人类家庭的绘图同样利用了一些相同的特征 为重组近交系建立的想法。 玉米 因此，这是一个模型系统，其中数量性状基因座可以更多地 易于绘制和表征。