GENE MAPPING AND GENOME ORGANIZATION IN MAMMALS

特定染色体基因标记的准确定位 染色体变化的片段和定量分析 在哺乳动物进化过程中需要对这些标记进行定位 为了描述哺乳动物进化的组织是 需要用来描述哺乳动物基因组的组织。 老鼠将被用作分析基因定位的模型， 澄清特定染色体片段上的基因顺序，以及 共性和连结守恒模式的特征 在哺乳动物进化的过程中。这些研究需要汇编和 小鼠连锁数据和哺乳动物基因同源性数据的分析。 正在开发两种计算机资源，完成后， 将包括(1)原始小鼠基因图谱数据和分析 程序(MLDP、鼠标连接数据库和程序)，以及(2) 哺乳动物基因同源性数据和分析程序(HMDP，同源性 数据库和程序)。MLDP和HMDP都将得到维护和 作为拟议分析的资源和研究工具得到加强 对于科学界(目标1)。小鼠基因图谱数据 MLDP中的分析程序将用于计算和 比较重组频率和映射距离，确定 可选择的基因序列的可能性，并创造节段性 代表基因定义明确的染色体片段的图谱 (目标2)。鼠标联动测试十字将用于解析 小鼠中位置未知的基因的基因顺序 对于小鼠中可能保守的部分基因 哺乳动物的染色体片段(目标3)。哺乳动物的同步性和 将使用HMDP中的连锁同源性数据和分析程序 用来估计个体的共性守恒程度 哺乳动物的血统和测试同步化的速度 不同血统之间的破坏差异很大(目标4)。这些 拟议的研究代表了一项全面的管理计划 小鼠基因作图数据和哺乳动物基因同源数据 利用这些数据研究人类基因组的组织和进化 哺乳动物。

Accurate localization of gene markers for specific chromosomal segments and quantitative analyses of changes in chromosomal localization of these markers during mammalian evolution are needed to characterize the organization of the mammalian evolution are needed to characterize the organization of the mammalian genome. The mouse will be used as a model for analyzing gene location, clarifying gene order on specific chromosomal segments, and characterizing the patterns of synteny and linkage conservation during mammalian evolution. These studies require compilation and analysis of mouse linkage data and mammalian gene homology data. Two computer resources are being developed and that, when complete, will consist of (1) raw mouse gene mapping data and analysis programs (MLDP, mouse linkage database and programs), and (2) mammalian gene homology data and analysis programs (HMDP, homology database and programs). Both MLDP and HMDP will be maintained and enhanced as a resource and research tool for the proposed analyses and for the scientific community (Aim 1). Mouse gene mapping data and analytical programs in MLDP will be used for calculating and comparing recombination frequencies and map distances, determining the likelihood for alternative gene orders, and creating segmental maps that represent genetically well-defined chromosomal segments (Aim 2). Mouse linkage testing crosses will be used for resolving gene order both for genes whose location is uncertain in the mouse and for genes in the mouse that are part of potentially conserved chromosomal segments among mammals (Aim 3). Mammalian synteny and linkage homology data and analytical programs in HMDP will be used for estimating the extent of synteny conservation in individual mammalian lineages and for testing whether rates of synteny disruption vary significantly among lineages (Aim 4). These proposed studies represent a comprehensive program for managing mouse gene mapping data and mammalian gene homology data and for using these data to study genome organization and evolution in mammals.