CARBOHYDRATE AND FSH SPECIFICITY DETERMINATION

本研究的总体目标是了解结构-功能 促性腺激素的关系。 促性腺激素，促黄体激素 促卵泡激素（FSH）和绒毛膜促性腺激素（LH） 促性腺激素（CG）在生殖调节中起着重要作用。 促性腺激素用于治疗不孕症，以确定 排卵，并指示人类怀孕。 此外，人类CG 在某些癌症中被用作肿瘤标志物。 实验模型 促性腺激素的作用是基于这些激素在大鼠中的作用。 一 在这些研究中，FSH的常用替代品是马绒毛膜促性腺激素 促性腺激素 eCG的主要结构牢固地确立了它作为一个 LH/CG是糖蛋白激素家族的成员，但已知它具有 许多生物测定中的FSH活性。 马LH共享相同的蛋白质 具有eCG结构，也具有FSH活性。 比较 eCG和ELH刺激大鼠颗粒细胞的活性 揭示了他们对这些激素的反应的差异。 自从eLH 和eCG的区别仅在于它们的碳水化合物部分，这表明， 这些细胞正在检测碳水化合物结构的差异。 具体目标#1是表征α亚基的作用 寡糖刺激颗粒细胞反应。 一系列 由eLH、eCG和 EFSH亚基将被制备，并且它们刺激 将比较eCG和DES引发的颗粒细胞中的类固醇生成。 这个实验将证明阿尔法的差异 低聚糖亚基是导致反应差异的原因 每一种激素的分泌。 eLH和eCG与 将表征大鼠卵巢的冷冻切片。 具体目标#2 是研究寡糖在靶细胞活化中的作用。 从eLH、eCG、EFSH和EFSH的α亚基纯化的寡聚腺苷酸 将比较垂体游离α，以确定 每个激素上的α亚基寡糖群。 的 寡糖将在颗粒细胞测定系统中进行检测， 以确定它们是否会干扰对FSH的反应 刺激.

The overall goal of this research is to understand the structure-function relationships of the gonadotropins. The gonadotropins, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and chorionic gonadotropin (CG), play a central role in the regulation of reproduction. Gonadotropins are used to treat infertility, to determine the time of ovulation, and to indicate pregnancy in humans. In addition, human CG is used as a tumor marker in certain cancers. Experimental models of gonadotropin action are based on action of these hormones in rats. A frequent substitute for FSH in these studies has been equine chorionic gonadotropin. The primary structure of eCG firmly establishes it as an LH/CG member of the glycoprotein hormone family, yet it is known to have FSH activity in many bioassays. Equine LH shares identical protein structure with eCG and also has FSH activity. Comparison of the activities of eCG and ELH in stimulating eCG-primed rat granulosa cells has revealed differences in their response to these hormones. Since eLH and eCG differ only in their carbohydrate moieties, this suggests that the cells are detecting these differences in carbohydrate structure. Specific aim #1 is to characterize the role of the alpha subunit oligosaccharides in stimulation of granulosa cell response. A series of hybrid hormones consisting of all possible combinations of eLH, eCG, and EFSH subunits will be prepared and their abilities to stimulate steroidogenesis in eCG- and DES-primed granulosa cells will be compared. This experiment will demonstrate whether or not differences in alpha subunit oligosaccharides are responsible for the differences in response to each hormone by these granulosa cells. The binding of eLH and eCG to frozen sections of rat ovaries will be characterized. Specific aim #2 is to investigate the role of oligosaccharides in target cell activation. Oligosaccharides purified from alpha subunits of eLH, eCG, EFSH, and pituitary free-alpha will be compared to determine the differences in populations of alpha subunit oligosaccharides on each hormone. The oligosaccharides will be tested in the granulosa cell assay systems in order to determine if they can interfere with the response to FSH stimulation.