权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of a Novel, Safe Method for the Non-invasive Assessment of Human Bone Quality, In Vivo, using spatially offset Raman spectroscopy.

使用空间偏移拉曼光谱开发一种新颖、安全的体内人体骨骼质量非侵入性评估方法。

基本信息

批准号：
EP/H002693/1
负责人：
Allen Goodship
金额：
$ 212.59万
依托单位：
University College London
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2010
资助国家：
英国
起止时间：
2010 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FH002693%2F1
关键词：
Development Novel Safe Method Non

项目摘要

The proposed activity is part of an exciting programme of international and multi-disciplinary work developing Raman spectroscopy of skeletal tissues for clinical use; measured for the first time through the unbroken skin. We are initially targeting bone in patients with osteoporosis, but this novel technology is applicable to other tissues and conditions. This project will add value to our work with three major associated developments: optimisation of bone spectroscopy through skin (both temporally and spatially-resolved techniques); identification of major spectral features that are associated with bone strength; and improving fracture risk predictions in comparison to the current standard DXA scans. A major problem our Society faces as a consequence of continual advances in healthcare and increasing wealth is enhanced life expectancy. With this the ability to enjoy a healthy and active ageing is often compromised by painful long lasting degenerative diseases of the musculoskeletal system. Indeed the Government is keen on prevention through ealry diagnosis and lifestyle changes, health ageing is a National Government priority.Current techniques used to identify the major degenerative skeletal diseases such as osteoarthritis and osteoporosis are based on imaging using X-ray radiation (DXA scanning, CT scans and Radiography). These technologies are limited as they rely on imaging the mineral component of the bone tissue, the mechanical strength of bone,however, is determined largely by the protein component, predominantly collagen type I and this is invisible to X-ray techniques.Bone tissue is a composite material comprising an inorganic mineral crystal component and an organic proteinaceous fibre component. The mineral fraction largley determines the stiffness of the material and the fibre component the strength.Many bone diseases and age related changes to the skeleton arise as a consequence of changes in the protein chemistry, either as a consequence of the genetic make up of an indivudual or the mechanical loading of the bone itself.Recently our collaborative team has developed a new revolutionary technique capable of determining bone tissue composition non-invasively through patients' skin. The concept, Spatially Offset Raman Spectroscopy (SORS), enables safe characterisation of tissue in vivo at depths of several millimetres. This is by at least an order of magnitude deeper than that possible with conventional Raman methods. Our breakthrough paves the way for the development of safe techniques for the early diagnosis of diseases where subtle molecular details within tissue can provide indicators of health problems, for example, the non-invasive diagnosis of genetic bone disease such as brittle bone disease and degenerative conditions such as arthritis. Other analytical applications include the probing of pharmaceutical products in depth and through coatings and packaging, the subsurface probing of paints, food products and security screening. The research project is enhanced through a strong cross-disciplinary science component opening a new branch of Raman spectroscopy with strong potential for commercial exploitation of research outcomes. Our research has permitted us to obtain the first Raman spectra of human bone in vivo under totally safe conditions (2 mW) as a basic proof of concept. Although as yet unoptimised the available information can be contrasted with that obtainable using conventional X-ray techniques such as DEXA which only yield the density of the mineral component. The extra information contained in Raman spectra holds promise for the characterisation of bone diseases such as osteoporosis or brittle bone disease. To realise this goal, further research is now needed to increase the penetration depth and establish a firm link between Raman spectra and specific bone diseases. This proposal has the potential to develop a new diagnostic modality in modern medicine.

拟议的活动是一项令人兴奋的国际和多学科工作计划的一部分，该计划旨在开发用于临床的骨骼组织拉曼光谱;首次通过未破损的皮肤进行测量。我们最初针对骨质疏松症患者的骨骼，但这项新技术适用于其他组织和疾病。该项目将通过三个主要的相关发展为我们的工作增加价值：通过皮肤优化骨光谱（时间和空间分辨技术）;识别与骨强度相关的主要光谱特征;与当前标准DXA扫描相比，改善骨折风险预测。由于医疗保健的不断进步和财富的不断增加，我们社会面临的一个主要问题是预期寿命的延长。因此，享受健康和积极老龄化的能力往往受到肌肉骨骼系统疼痛的长期退行性疾病的影响。事实上，政府热衷于通过早期诊断和改变生活方式进行预防，健康老龄化是国家政府的优先事项，目前用于识别骨关节炎和骨质疏松症等主要退行性骨骼疾病的技术是基于X射线辐射成像（DXA扫描，CT扫描和放射照相术）。这些技术是有限的，因为它们依赖于对骨组织的矿物质成分成像，然而，骨的机械强度主要由蛋白质成分（主要是I型胶原）决定，而这对于X射线技术是不可见的。矿物质成分在很大程度上决定了材料的硬度，纤维成分决定了材料的强度。许多骨骼疾病和骨骼的年龄相关变化都是由于蛋白质化学变化的结果，无论是作为一个人的遗传组成或骨本身的机械负荷的结果。最近，我们的合作团队已经开发出一种新的革命性的技术，能够确定骨组织组成非侵入性地穿过病人的皮肤。空间偏移拉曼光谱（SORS）的概念，使在几毫米的深度在体内组织的安全表征。这比常规拉曼方法可能的深度至少深一个数量级。我们的突破为开发用于早期诊断疾病的安全技术铺平了道路，其中组织内的细微分子细节可以提供健康问题的指标，例如，遗传性骨病（如脆骨病）和退行性疾病（如关节炎）的非侵入性诊断。其他分析应用包括药品的深度探测、涂层和包装探测、油漆、食品和安全检查的表面下探测。该研究项目是通过一个强大的跨学科的科学组成部分，打开一个新的分支的拉曼光谱与强大的潜力，为商业开发的研究成果。我们的研究使我们能够获得第一个拉曼光谱的人骨在体内完全安全的条件下（2毫瓦）作为一个基本的概念证明。尽管尚未优化，但可用的信息可以与使用常规X射线技术（例如DEXA）获得的信息进行对比，常规X射线技术仅产生矿物组分的密度。拉曼光谱中包含的额外信息有望用于骨质疏松症或脆骨病等骨骼疾病的表征。为了实现这一目标，现在需要进一步的研究来增加穿透深度，并在拉曼光谱和特定的骨骼疾病之间建立牢固的联系。这一建议有可能在现代医学中开发一种新的诊断模式。